Applications
Extraction of Barbiturates from Urine on Strata™ X
14622
Separation Mode: Reversed Phase
Reversed Phase
Max-RP
HPLC
Adjuvants, Anesthesia
Forensics/Toxicology
Pharmaceutical
Therapeutic / Clinical

Applications

Extraction of Barbiturates from Urine on Strata™ X

Analytes

1
2
3
4
5
Compound Name
Phenobarbital
CID
4763
Molecular Formula
C12H12N2O3
Molecular Weight
232
No. Hydrogen Bond Acceptors
3
No. Hydrogen Bond Donors
2

Conditions

LC Conditions (App ID: 14622)
Column:Synergi 4 µm Max-RP 80 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Synergi
Part No:00F-4337-E0
Elution Type:Gradient
Mobile Phase:A: 20 mM KH2PO4, pH 7.0
B: Acetonitrile
Gradient Profile:
Step No.Time(min)%A%B
108020
238020
3155050
4168020
Flow Rate:1 mL/min
Temperature:22°C
Detection:UV-Vis Abs.-Fixed Wave. (UV) nm @ 22°C

Sample Preparation Details
Description:Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk
Part Number:8B-S100-TAK
Pre Treatment Note:Blow down under slow stream of N2 @ 40° C and reconstitute with 300 µL water
SPE Condition 1:1.0 mL Methanol
SPE Condition 2:1.0 mL Water
SPE Load:Load 1.00 mL Sample diluted in KH2PO4 (100mM) pH 6.0
SPE Wash 1:1.0 mL Methanol / Water (5:95)
SPE Wash 2:1.0 mL Methanol / 2% CH3COOH in Water (10:90)
SPE Elution 1:1.0 mL Methanol / 2% Ammonium Hydroxide in Water (35:65)
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:300 µL Water

Order Items Used in This Application

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