Applications
Extraction of Diazepam on Strata™ X
14618
Separation Mode: Reversed Phase
Reversed Phase
Polar-RP
HPLC
Antiemetics
Toxicology / Drug Screening / TDM
Therapeutic / Clinical

Applications

Extraction of Diazepam on Strata™ X
Analytes
1
2
Compound Name
Nordiazepam
Details
LC Conditions (App ID: 14618)
Column:
Brand Name:
Part No:
Elution Type:
Gradient
Mobile Phase:
A: 20 mM KH2PO4 (pH=2.5)
B: MeOH
Gradient Profile:
Step No.Time(min)%A%B
107525
237525
383070
4113070
5147525
Flow Rate:
1.5 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C
Sample Preparation Details
Description:
Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk
Part Number:
Pre Treatment Note:
Reconstitute with 200 µL mobile phase
SPE Condition 1:
1.0 mL Methanol
SPE Condition 2:
1.0 mL Water
SPE Load:
Load 1000.00 µL Sample
SPE Wash 1:
1.0 mL Methanol:Water
SPE Dry:
1 min @ 10 in. Hg
SPE Elution 1:
Methanol
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
200 µL Mobile Phase

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