Applications
Extraction of Diazepam on Strata™ X
14618
Separation Mode: Reversed Phase
Reversed Phase
Polar-RP
HPLC
Antiemetics
Toxicology / Drug Screening / TDM
Therapeutic / Clinical

Applications

Extraction of Diazepam on Strata™ X

Analytes

1
2
Compound Name
Nordiazepam
CID
2997
Molecular Formula
C15H11ClN2O
Molecular Weight
270
No. Hydrogen Bond Acceptors
2
No. Hydrogen Bond Donors
1

Conditions

LC Conditions (App ID: 14618)
Column:Synergi 4 µm Polar-RP 80 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Synergi
Part No:00F-4336-E0
Elution Type:Gradient
Mobile Phase:A: 20 mM KH2PO4 (pH=2.5)
B: MeOH
Gradient Profile:
Step No.Time(min)%A%B
107525
237525
383070
4113070
5147525
Flow Rate:1.5 mL/min
Temperature:22°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

Sample Preparation Details
Description:Strata-X 33 µm Polymeric Reversed Phase, 30 mg / 1 mL, Tubes, 100/Pk
Part Number:8B-S100-TAK
Pre Treatment Note:Reconstitute with 200 µL mobile phase
SPE Condition 1:1.0 mL Methanol
SPE Condition 2:1.0 mL Water
SPE Load:Load 1000.00 µL Sample
SPE Wash 1:1.0 mL Methanol:Water
SPE Dry:1 min @ 10 in. Hg
SPE Elution 1:Methanol
SPE Evaporation:Evaporate to dryness
SPE Reconstitute:200 µL Mobile Phase

Order Items Used in This Application

Similar Applications
No Data