Applications
Steroids on Strata™ X
14617
Anti-Inflammatory Agents
Clinical
Pharmaceutical/Biopharmaceutical
Pharmaceutical
Therapeutic / Clinical

Applications

Steroids on Strata™ X
Analytes
1
2
3
4
Compound Name
Prednisolone
CID
5755
Molecular Formula
C21H28O5
Molecular Weight
360
No. Hydrogen Bond Acceptors
5
No. Hydrogen Bond Donors
3
Details
LC Conditions (App ID: 14617)
Elution Type:
Isocratic
Mobile Phase:
A: 20 mM Potassium Phosphate, pH 7
B: Methanol
Gradient Profile:
Step No.Time(min)%A%B
103565
Flow Rate:
1 mL/min
Temperature:
22°C
Detection:
UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C
Sample Preparation Details
Pre Treatment Note:
Add 25 mL of 0.1 mg/ml butyl paraben (I.S.). 40 °C under N2 stream and reconstitute in 200 µL Acetonitrile
SPE Condition 1:
1.0 mL Methanol
SPE Condition 2:
1.0 mL Water
SPE Load:
Load 1.00 mL Sample
SPE Wash 1:
1.0 mL Methanol:Water
SPE Dry:
1 min @ 10 in. Hg
SPE Elution 1:
1.0 mL Methanol
SPE Evaporation:
Evaporate to dryness
SPE Reconstitute:
200 µL Acetonitrile