Applications
Nucleic acids
12486
Separation Mode: Reversed Phase
Reversed Phase
Polar-RP
HPLC
Biochemical - NOT Polymeric (incl. oligomers)
Biochemical Compound / Nutrient

Applications

Nucleic acids

Analytes

1
2
3
4
Compound Name
Guanine
CID
764
Molecular Formula
C5H5N5O
Molecular Weight
151
No. Hydrogen Bond Acceptors
4
No. Hydrogen Bond Donors
3

Conditions

LC Conditions (App ID: 12486)
Column:Synergi 4 µm Polar-RP 80 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Synergi
Part No:00F-4336-E0
Elution Type:Isocratic
Mobile Phase:A: 20mM Potassium phosphate pH 2.7
Gradient Profile:
Step No.Time(min)%A
10100
Flow Rate:2 mL/min
Temperature:22°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

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