Applications
Nicotine and Related Compounds
10901
Separation Mode: Reversed Phase
Reversed Phase
Max-RP
HPLC
Uncoupling Agents
Clinical
Pharmaceutical
Therapeutic / Clinical

Applications

Nicotine and Related Compounds

Analytes

1
2
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7
Compound Name
Cotinine-N-Oxide
CID
108114
Molecular Formula
C10H12N2O2
Molecular Weight
192
No. Chiral Centers:
1
No. Hydrogen Bond Acceptors
2

Conditions

LC Conditions (App ID: 10901)
Column:Synergi 4 µm Max-RP 80 Å, LC Column 150 x 4.6 mm, Ea
Brand Name:Synergi
Part No:00F-4337-E0
Elution Type:Gradient
Mobile Phase:A: 10 mM Ammonium acetate in water
B: 10 mM Ammonium acetate in methanol
Gradient Profile:
Step No.Time(min)%A%B
109010
20.259010
33.750100
Flow Rate:1 mL/min
Temperature:22°C
Detection:UV-Vis Abs.-Variable Wave.(UV) nm @ 22°C

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