Product Information
Technique
Size Exclusion Chromatography (SEC)
Size exclusion is a technique that separates target molecules based on their size. It can be used for the analysis of polymer, proteins, and peptides.
Size Exclusion Chromatography (SEC)

Size Exclusion Chromatography (SEC) is a chromatographic method which separates analytes solely based on their size, where molecules are separated on the basis of their exclusion from pores in the column packing material. Larger analytes will elute first, while the smaller molecules interact more with the stationary phase and will elute later.

Based on the analytes and the mobile phases used, size exclusion chromatography can be broken into two main categories, aqueous size exclusion/gel filtration chromatography (GFC) and non-aqueous size exclusion/gel permeation chromatography (GPC).

Aqueous Size Exclusion/ Gel Filtration Chromatography (GFC)
Analyze and/or characterize proteins, peptides, and other biomolecules such as antibodies, immunoglobulins, protein complexes, protein aggregates, and desalting.

Non-Aqueous Size Exclusion/ Gel Permeation Chromatography (GPC)
Analyze polymers such as adhesives, oils, plasticizers, plastics, resins, and rubbers/elastomers.


SEC Column

Brand Description:
bioZen SEC-2 and SEC-3 phases provide high efficiency separation for biomolecules
Phenogel Non-aqueous gel permeation columns (GPC) that provide high separation for polymers
Yarra High speed gel filtration columns (GFC) for biomolecules