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Technique
HILIC HPLC: Phenomenex
HILIC provides a unique separation for polar compounds that cannot be effectively separated by reversed phase
HILIC HPLC

HILIC is a particularly useful separation mode for polar organic compounds that are poorly retained by reversed phase. Retention of these polar compounds using reversed phase methods is often difficult because of co-elutions with the solvent front or elutions within the chromatographic region where ion suppression is the greatest.

HILIC HPLC/UHPLC columns draw and retain a water-enriched layer onto the surface of the silica which facilitates the interaction of polar compounds with the stationary phase for increased retention.

HILIC HPLC Phase
Brand Description:
Kinetex HILIC Novel core-shell unbonded silica phase provides significant selectivity for retention and separation of polar compounds
Luna HILIC Offers excellent MS sensitivity and selectivity for polar compounds