Gel filtration chromatography (GFC) is used to separate large macromolecules such as antibodies, immunoglobulins, protein complexes, protein aggregates, peptides, and other biomolecules. In gel filtration chromatography, the compounds of interest “filter” through the stationary phase based on their size.
Typically aqueous solvents are used in the mobile phase to ensure that the compound of interest maintains biological activity. Gel filtration columns can separate biomolecules that range from 200 to 1,500,000 Daltons in size. Gel filtration chromatography columns require a GFC column that has low surface activity, high efficiency, and consistent uniform pore size.