Product Information
Phase Detail
Zebron ZB-Bioethanol GC Columns
Zebron ZB-bioethanol GC columns are designed for rapid bioethanol testing
Zebron ZB-Bioethanol GC Columns
Fast Quality Checks for Bioethanol Testing

  • Specially designed for fast and accurate bioethanol analysis
  • Meet ASTM D5501 requirements - resolve methanol and ethanol from all other denaturant peaks
  • Great resolution of fusel alcohols
  • Allows for quick bake out between runs to eliminate contaminants

Doing biodiesel testing? See ZB-1HT or ZB-5HT.

Recommended Use
Alcohol, Bioethanol, Fusel Alcohols

 

 

R&D 100 Award-Winning
Technology for High Temp Testing with advanced polymer bonding technology and specialized high temperature polyimide coating, Zebron Inferno columns offer enhanced performance where other columns fail. See how Inferno columns allow analysts to push a standard analysis up to 430 °C. Download the brochure here.


zbBioEnthanol

Temperature Limits
-60 to 340/360
Phase
ZB-Bioethanol
Recommended Use
Meets ASTM D5501 requirements and resolves methanol and ethanol from all other denaturant peaks
Upgrade to Zebron from traditional phases used for bioethanol:

Agilent® Restek® SGE® Supelco®
DB®-1
HP-1
CP-Sil 5 CB
Rtx®-1
Rxi®-1ms
BP1 SPB®-1
SE-30

Zebron ZB-Bioethanol
  • Resolve methanol and ethanol from all other denaturant peaks
  • With analysis time down to 5 minutes, complete certificate of analysis (COA) before fuel truck leaves
  • Major cost savings over the standard 100 meter GC columns
Zebron ZB-Bioethanol columns provide bioethanol analysis in less than half the time of standard GC columns. Using this new, specially designed phase, resolution of methanol and ethanol from a sample denatured with gasoline can be achieved in less than 8 minutes. In fact, the column provides such great resolution that the analysis can be reduced to less than 5 minutes by using a 15 meter column. This dramatic time savings gives you the ability to test the fuel trucks before they leave the facility reducing the chance for potential problems.

ASTM Method D 5501: Determination of Denatured Bioethanol

Column:
Zebron ZB-1
Dimension:
A) 100 meter x 0.25 mm x 0.50 µm
Part No.:
7MG-G001-17
Injection:
Split 50:1 @ 300 °C, 1 µL
Carrier Gas:
Helium @ 35 cm/sec (Constant Flow)
Oven Program:
45 °C for 7 min to 255 °C @ 30 °C/min
(Hold 6 min)
Detector:
FID @ 300 °C
Instrument:
Shimadzu® GC-2010 with Flame Ionization Detection and AOC-20i Automatic Liquid
Sample:
1. Methanol
2. Ethanol
3. Denaturant

ASTM Method D 5501: Determination of Denatured Bioethanol

Conditions for both columns:

Column:
Zebron ZB-Bioethanol
Dimension:
B) 30 meter x 0.25 mm x 1.00 µm
C) 15 meter x 0.25 mm x 1.00 µm
Part No.:
B) 7HG-G020-22
C) 7EG-G020-22
Injection:
Split 50:1 @ 300 °C, 1 µL
Carrier Gas:
B) Hydrogen @ 35 cm/sec (Constant Flow)
C) Hydrogen @ 25 cm/sec (Constant Flow)
Oven Program:
B) 45 °C for 2.5 min to 255 °C @ 30 °C/min (Hold 4.5 min)
C) 55 °C for 1.7 min to 260 °C @ 40 °C/min (Hold 2.67 min)
Detector:
FID @ 300 °C
Instrument:
Shimadzu® GC-2010 with Flame Ionization Detection and AOC-20i Automatic Liquid
Sample:
1. Methanol
2. Ethanol
3. Denaturant

Great Resolution of Fuel Alcohols Products

Since gasoline is a very complex mixture and contains many low boiling components that could potentially co-elute with methanol and ethanol, the resolution provided by the Zebron™ ZB-Bioethanol column was confirmed using GC-MS. As you can see, the methanol and ethanol peaks are well resolved from all potential interferences.

Column:
Zebron ZB-Bioethanol
Dimension:
30 meter x 0.25 mm x 1.00 μm
Part No.:
7HG-G020-22
Injection:
Split 100:1 @ 240 °C, 0.1 μL
Carrier Gas:
Helium @ 1.2 mL/min (Constant Flow)
Oven Program:
40 °C for 5 min to 300 °C @ 25 °C/min
Detector:
MSD @ 230 °C; 30 - 450 amu

Sample:
1. Methanol
2. Ethanol
3. Acrolein
4. Acetone
5. 2-Methylbutane
6. Isopropyl alcohol
7. Pentane
8. t-Butanol
9. Allyl alcohol
10. n-Propanol
11. 2,3-Dimethylbutane
12. 2-Methylpentane
13. 3-Methylpentane
14. 2-Butanol
15. Ethyl acetate
16. Hexane
Sample:
17. Methylcyclopentane
18. 2,4-Dimethylpentane
19. Benzene
20. Cyclohexane
21. 2-Methylhexane
22. 2,3-Dimethylpentane
23. 3-Methylhexane
24. 2,2,4-Trimethylpentane
25. Heptane
26. Acetal
27. Toluene
28. Xylene
29. Trimethylbenzene
Compatible with Various Carrier Gases

As competition in the biofuels market increases, the need to reduce costs while maintaining quality is of major concern. The shortages of helium and its increasing costs have forced many labs to consider using alternative gases such as hydrogen.

The Zebron™ ZB-Bioethanol column is compatible with all carrier gases and will provide great separation no matter which gas you choose. To verify this, we ran several denatured fuel ethanol samples using various carrier gases. The results were virtually the same regardless of the carrier gas, proving the selectivity provided by the Zebron™ ZB-Bioethanol was not affected.

No resolution changes using different carrier gases

Conditions for both columns:

Column:
Zebron ZB-Bioethanol
Dimension:
15 meter x 0.25 mm x 1.00 μm
Part No.:
7EG-G020-22
Injection:
Split 50:1 @ 300 °C, 1 μL
Carrier Gas:
A) Hydrogen @ 25 cm/sec (Constant Flow)
B) Helium @ 25 cm/sec (Constant Flow)
Oven Program:
55 °C for 1.7 min to 260 °C @ 40 °C/min (Hold 2.67 min)
Detector:
FID @ 300 °C
Instrument:
Shimadzu® GC-2010 with a AOC-20i
Automatic Liquid Handler
Sample:
1. Methanol
2. Ethanol
3. Denaturant