Product Information
Phase Detail
Zebron ZB-WAX<em>PLUS</em> GC Columns
Zebron ZB-WAXPLUS GC column is 100% aqueous stable and is great for low boiling point solvents and chlorinated solvents

Zebron ZB-WAXPLUS GC Columns

100% Aqueous Stability for Polar Compounds

  • 100% aqueous stable, excellent for aqueous samples; bonded and solvent rinseable
  • Extremely inert for acidic compounds
  • Enhanced selectivity for low boiling solvents; high retention of alcohols and chlorinated solvents
  • Increased efficiency at 20 °C
Need a G43 phase for residual solvents testing? See ZB-624.
Testing free fatty acids? See ZB-FFAP.

Recommended Applications
Alcohols, Aldehydes, Aromatics, Essential Oils, Flavors & Fragrances, Free Fatty Acids, Glycols, OVIs, Pharmaceuticals, Solvents / Residual Solvents, Styrene, Xylene Isomers

zbWAXplus

Temperature Limits
20 to 250/260
Phase
ZB-WAXplus
Recommended Use
Enhanced selectivity for low boiling solvents; high retention of alcohols and chlorinated solvents and extremely inert for acidic compounds

Upgrade to Zebron from any polyethylene glycol phase:

Agilent® Restek® SGE Supelco®
DB®-WAX
CAM
HP-20M
Carbowax 20M
CP-Wax 52 CB
Stabilwax® BP20 SUPELCOWAX® 10

Zebron ZB WAXPLUS GC Column

WAX columns from different manufacturers are not always created equal!

As part of the Zebron PLUS GC column family, ZB-WAXPLUS is manufactured using improved technologies that reflect our attention to detail every aspect of a ZB-WAXPLUS column’s anatomy is meticulously engineered and individually tested for unprecedented performance.

 


Water Reproducibility of ZB-WAXPLUS

Historically, polyethylene glycol (PEG) phases have been unstable with aqueous samples such as beverages or glycols, resulting in poor reproducibility and decreased lifetime. The ZB-WAXplus bonding procedure results in exceptional stability to repeated injections of aqueous matrices.

Reproducibility and Stability Test

Columns:
Zebron ZB-WAXPLUS
Dimension:
30 meter x 0.25 mm x 0.25 µm
Part No.:
7HG-G013-11
Injection:
Split 30:1 @ 140 °C, 0.2 µL
Carrier Gas:
Helium @ 1.4 mL/min (constant flow)
Oven Program:
35 °C for 5 min to 85 °C @ 10 °C/min to 200 °C @ 25 °C/min for 1 min
Detector:
FID @ 200 °C
Sample:
1. Acetaldehyde
2. Ethyl Acetate
3. Methanol
4. Ethanol
5. Propanol
6. Isobutanol
7. 2-Methylbutanol
8. 3-Methylbutanol

ZB-WAXPLUS provides optimal selectivity for many aqueous soluble compounds such as those found in alcoholic beverages or glycol samples. The ZB WAXPLUS bonding process results in exceptional stability to repeated injections of aqueous matrices like whiskey. Multiple or routine water injections will not alter the efficiency or polarity of the phase over time.

Distilled Alcohol Standard by Neat Injection on GC-FID

Columns:
Zebron ZB-WAXPLUS
Dimension:
30 meter x 0.25 mm x 0.25 µm
Part No.:
7HG-G013-11
Injection:
Split 25:1 @ 210 °C, 1 µL
Carrier Gas:
Hydrogen @ 1 mL/min (constant flow)
Oven Program:
35 °C for 6 min to 60 °C @ 5 °C/min for 2 min to 210 °C @ 10 °C/min
Detector:
FID @ 230 °C
Note:
200 ppm standard in methylene
chloride

Sample:
1. Acetaldehyde
2. Isobutanal
3. Ethyl formate
4. Acrolein
5. Ethyl acetate
6. Acetal
7. Methanol
8. Methylene chloride
9. Ethanol
10. Isobutyl acetate
11. 2-Butanol
12. Ethyl butyrate
13. 1-Propanol
14. Isobutanol
15. Allyl alcohol
16. Isoamyl acetate
17. 1-Butanol
18. 4-Methyl-2-pentanol
Sample:
19. Methyl-2-butanol
20. Methyl-3-butanol
21. Ethyl caproate
22. Ethyl heptanoate
23. Ethyl lactate
24. Hexanol
25. cis-3-Hexenol
26. Ethyl caprylate
27. Furfural
28. Benzaldehyde
29. Linalool
30. Linalyl acetate
31. Ethyl caprate
32. Diethyl succinate
33. Ethyl laurate
34. 2-Phenyl ethanol

Don’t Despair – There’s a Solution!

Low column activity is essential to achieving good quantitation and sensitivity. Whether you are analyzing aldehydes, acids, or pharmaceutical compounds the ZB-WAXPLUS provides the highest level of deactivation possible.

Comparative separations may not be representative of all applications.
Conditions same for both columns:

Dimension:
30 meter x 0.25 mm x 0.25 μm
Part No.:
7HG-G013-11(ZB-WAXPLUS
Injection Volume:
Split 100:1 @ 250 °C, 1 μL
Carrier Gas:
Hydrogen @ 1.0 mL/min (constant flow)
Oven Program:
40 °C for 5 min to 200 °C at 10 °C/min and hold until last peak elutes
Detector:
FID @ 225 °C
Sample:
1. Propanal
2. Butanal
3. Pentanal
4. Hexanal
5. Heptanal
6. Octanal
7. Nonanal
8. Decanal
9. Undecanal

Holds Up To Tough Conditions

In addition to inertness, ZB-WAXPLUS columns maintain good performance with difficult matrices and contaminants! The example below shows good results with glycols from seawater, despite the salt and other contaminants commonly found in this matrix.

Glycols From Seawater By GC-MS

Analytical Procedure

Sample Preparation

SPE Sorbent:
Strata®-X-A 500 mg / 6 mL
Condition:
3 mL Methanol, 3 mL Water
Part No.:
8B-S123-HCH
Load:
1 mL seawater
(basified with ~25 µL of NH4OH)
Dry:
Full vacuum 5-10 minutes
Elute:
3 mL or 2 % Formic acid in Methanol


GC/MS Conditions

Column:
Zebron ZB-WAXPLUS
Dimension:
30 meter x 0.25 mm x 0.25 µm
Part No.:
7HG-G013-11
Injection:
Split 10:1 @ 250 °C, 1 µL
Carrier:
Helium @ 1.5 mL/min (constant flow)
Oven Program:
100 °C to 200 °C @ 20 °C/min, post run 5 min @ 250 °C
Detection:
MSD, SIM from 1-2.8 min mass 87 and 89, 2.8-5 min mass 31
 
Sample:
Analytes were extracted from
seawater (1 mL load @ 5 ppm)
1. D4-2-Butoxyethanol (IS)
2. 2-Butoxyethanol
3. Formic acid (from elution)
4. Propylene glycol
5. Ethylene glycol (IS)

Improve Resolution

Comparative separations may not be representative of all applications.
Conditions same for both columns:

Dimension:
30 meter x 0.25 mm x 0.25 µm
Part No.:
7HG-G013-11 (ZB-WAXPLUS)
Injection:
Split 100:1 @ 250 °C, 1 μL
Carrier:
Hydrogen @ 1.0 mL/min (constant flow)
Oven Program:
35 °C for 2.5 min to 85 °C @ 10 °C/min and hold until last peak elutes
Detector:
FID @ 225 °C
Sample:
1. Methyl Formate
2. Acetone
3. Ethyl Acetate
4. Methyl Ethyl Ketone
5. Methanol
6. 2-Methyl-2-propanol
7. Methylene Chloride
8. Benzene
9. Ethanol
10. 2-Butanol
11. Toluene
12. n-Propanol
13. Ethyl Benzene
14. p-Xylene
15. m-Xylene
16. 1-Butanol
17. o-Xylene

Faster analysis

ZB-WAXPLUS™ columns provide high efficiency with stable selectivity in a range of dimensions, so you can easily optimize your column dimensions and run up to 65 % faster.

Comparative separations may not be representative of all applications.

Conditions same for both columns:
Column:
Zebron ZB-WAXPLUS
Dimension:
As listed
Part No.:
A) 7KG-G013-11
B) 7CB-G013-02
Injection:
A) Split 40:1 @ 220 °C, 0.1 μL
B) Split 20:1 @ 220 °C, 0.2 μL
Carrier:
A) Helium @ 1.2 mL/min (constant flow)
B) Helium @ 0.3 mL/min (constant flow)
Oven Program:
A) 40 °C for 0.2 min to 210 °C @ 10 °C/min for 10 min
B) 35 °C for 1 min to 250 °C @ 30 °C/min for 5 min
Detection:
MSD; 45-450 amu

Sample:
1. α-Pinene
2. β-Pinene
3. Sabinene
4. 3-Carene
5. β-Myrcene
6. Limonene
7. β-Phellandrene
8. Octanal
9. Nonanal
10. Limonene Oxides
11. Citronellal
12. Decanal
13. α-Cubebene
14. Linalool
15. β-Cubebene
16. Octanol
 
17. Germacrene
18. Caryophyllene
19. trans-p-Mentha-2,8-dienol
20. cis-p-Mentha-2,8-dienol
21. Gerenial
22. α-Terpineol
23. Dodecanal
24. Valencene
25. Citral
26. Carvone
27. Cadinene
28. Perillaldehyde
29. trans-Carveol
30. cis-Carveol
31. Perillol
32. Octanoic acid