Product Information
Phase Detail
Zebron ZB-FAME GC Columns
Zebron ZB-FAME GC columns is designed for fatty acid methyl ester analysi
Zebron ZB-FAME GC Columns
The Fast FAME GC Column

  • Reduce run times for traditional FAMEs separations up to 75 %
  • Separate cis/trans FAME isomers with Rs ≥ 1.0
  • High-cyanopropyl selectivity acceptable for use with AOCS, AOAC, and IOC methods

Recommended Use
Fatty acid methyl esters (FAMEs), cis/trans FAME isomers, Omega 3 and Omega 6 FAMES

zbfame


Phase
ZB-FAME
Temperature Limits
20 to 250/260
Recommended Use
Resolution of fatty acid methyl esters (FAMEs) and cis/trans FAME isomers
Upgrade to Zebron from any high cyanopropyl phase:

Agilent® Supelco®
CP-Sil 88
HP-88
DB®-23
SP®-2380
SP-2560

Accelerating FAME Testing
Designed to move beyond the conventional to the exceptional , Zebron GC columns come to life through a coupling of innovative spirit and technical excellence. As part of the Zebron Unlimited series of columns, Zebron ZB FAME offers unparalleled performance through the power of targeted selectivity when analyzing fatty acid methyl esters (FAMEs)

Optimized High-Cyano Polarity
FAME separations require the use of high polarity columns for adequate resolution of cis/trans isomers. Zebron™ ZB-FAME is an optimized high-cyanopropyl (G48) chemistry that provides the polarity that you need, while remaining compatible with AOAC,  OCS, and IOC regulatory methods.

Elution Order for 37 FAME Standard

Peak
Compound
ID
1
Butanoic Acid Methyl Ester
C4:0
2
Hexanoic Acid Methyl Ester
C6:0
3
Octanoic Acid Methyl Ester
C8:0
4
Decanoic Acid Methyl Ester
C10:0
5
Undecanoic Acid Methyl Ester
C11:0
6
Dodecanoic Acid Methyl Ester
C12:0
7
Tridecanoic Acid Methyl Ester
C13:0
8
Myristic Acid Methyl Ester
C14:0
9
Myristoleic Acid Methyl Ester
C14:1 cis 9
10
Pentadecanoic Acid Methyl Ester
C15:0
11
cis-10-Pentadecenoic Acid Methyl Ester
C15:1 cis 10
12
Hexadecanoic Acid Methyl Ester
C16:0
13
Palmitoleic Acid Methyl Ester
C16:1 cis 9
14
Heptadecanoic Acid Methyl Ester
C17:0
15
cis-10-Heptadecenoic Acid Methyl Ester
C17:1 cis 10
16
Stearic Acid Methyl Ester
C18:0
17
Elaidic Acid Methyl Ester
C18:1 trans 9
18
Oleic Acid Methyl Ester
C18:1 cis 9
19
Linolelaidic Acid Methyl Ester
C18:2 trans 9,12
20
Linoleic Acid Methyl Ester
C18:2 cis 9,12

Peak
Compound
ID
21
γ-Linolenic Acid Methyl Ester
C18:3 cis 6,9,12
22
α-Linolenic Acid Methyl Ester
C18:3 cis 9,12,15
23
Arachidic Acid Methyl Ester
C20:0
24
cis-11-Eicosenoic Acid Methyl Ester
C20:1 cis 11
25
cis-11,14-Eicosadienoic Acid Methyl Ester
C20:2 cis 11,14
26
Heneicosanoic Acid Methyl Ester
C21:0
27
cis-8,11,14-Eicosatrienoic Acid Methyl Ester
C20:3 cis 8,11,14
28
Arachidonic Acid Methyl Ester
C20:4 cis 5,8,11,14
29
cis-11,14,17-Eicosatrienoic Acid Methyl Ester
C20:3 cis 11,14,17
30
Behenic Acid Methyl Ester
C22:0
31
Erucic Acid Methyl Ester
C22:1 cis 13
32
cis-5,8,11,14,17-Eicosapentaenoic Acid Methyl Ester
C20:5 cis 5,8,11,14,17
33
cis-13,16-Docosadienoic Acid Methyl Ester
C22:2 cis 13,16
34
Tricosanoic Acid Methyl Ester
C23:0
35
Lignoceric Acid Methyl Ester
C24:0
36
Nervonic Acid Methyl Ester
C24:1 cis 15
37
cis-4,7,10,13,16,19-Docosahexaenoic Acid Methyl Ester
C22:6 cis 4,7,10,13,16,19

QC Tested For FAMEs
Baseline Separation of Common Isomers
Food and dietary standards require separation of cis/trans fatty acids. Zebron™ ZB-FAME is able to separate from C4 to C24 with resolution of specific cis/trans fatty acids.

Completely Resolve AOAC Critical Pairs

Columns:
Zebron ZB-FAME
Dimension:
30 meter x 0.25 mm x 0.20 µm
Part No.:
7HG-G033-10
Injection:
Split 50:1 @ 240 °C, 1 µL
Recommended Liner:
Zebron PLUS Single Taper with Wool
Liner Part No.:
AG2-0A11-05 (for Agilent® systems)
Carrier Gas:
Helium @ 1.2 mL/min (constant flow)
Oven Program:
100 °C for 2 min to 140 °C @ 10 °C/min
to 190 °C @ 3 °C/min to 260 °C @ 30 °C/min for 2 min
Detector:
FID @ 260 °C
Sample:
37 FAME standard

Separate Detailed C18 Compounds
Due to regulatory guidelines around trans fat content in food, detailed analysis of specific FAMEs isomers is commonly performed. The below methods demonstrate this testing using a 60 meter Zebron™ ZB-FAME column.

Analysis of C18:1, C18:2, and C18:3

Columns:
Zebron ZB-FAME
Dimension:
60 meter x 0.25 mm x 0.20 µm
Part No.:
7KG-G033-10
Injection:
Split 50:1 @ 240 °C, 1 µL
Recommended Liner:
Zebron PLUS Single Taper with Wool
Liner Part No.:
AG2-0A11-05 (for Agilent® systems)
Carrier Gas:
Helium @ 30 cm/sec (constant flow)
Oven Program:
160 °C (isothermal)
Detector:
FID @ 260 °C
Sample:
As listed. For full peak identification, visit www.phenomenex.com/DetailedFAME

Twice The Runs, Half The Time
Traditionally, cis/trans FAME separations require the use of long (100 meters or more) columns and can run up to 60 minutes, resulting in a bottleneck to higher productivity. Zebron™ ZB-FAME provides targeted selectivity that allows for reduced column length – run times as short as 11 minutes without compromising your results!

37 FAMEs In A Short 11 Minute Run

Columns:
Zebron ZB-FAME
Dimension:
20 meter x 0.18 mm x 0.15 μm
Part No.:
7FD-G033-05
Injection:
Split 100:1 @ 250 °C, 1 µL
Recommended Linear:
Zebron PLUS Single Taper Z-Liner™
Liner Part No.:
AG2-0A13-05 (for Agilent® systems)
Carrier Gas:
Helium @ 1.0 mL/min (constant flow)
Oven Program:
80 °C for 1.5 min to 160 °C @ 40 °C/min to 185 °C @ 5 °C/min to 260 °C @ 30 °C/min
Detector:
FID @ 260 °C
Sample:
37 FAME standard