Product Information
Phase Detail
Zebron ZB-BAC-1 GC Columns
Zebron ZB-BAC-1 GC columns is ideal for the resolution of ethanol and acetone
Zebron ZB-BAC-1 GC Columns
Fast, Accurate Blood Alcohol Analysis

  • Proprietary column set specially designed for accurate blood alcohol analysis — enhance resolution of ethanol and acetone peaks
  • Resolves t-butanol and n-propanol for greater selection of internal standards
  • Fast run time with baseline resolution of key components in just 2 minutes
  • Achieve dual column confirmation - get two elution order changes with ZB-BAC-2

Need an alternate selectivity for blood alcohol confirmation? See ZB-BAC-2.  
Testing drugs of abuse? See ZB-Drug-1.

Recommended Use
Abused Inhalant Anesthetics, Blood Alcohol Analysis

zbBAC_1_2

Phase
ZB-BAC-1
Temperature Limits
-20 to 260/280
Recommended Use
Enhanced resolution of ethanol and acetone peaks for blood alcohol analysis and dual-column confirmation with two elution order changes when used with ZB-BAC-2
Upgrade to Zebron from these similar* phases:

Agilent® Restek®
DB®-ALC1
DB-ALC2
Rtx®-BAC1
Rtx-BAC2
Rtx-VMS

*not exact equivalent, selectivity may differ


Faster, More Sensitive Blood Alcohol Analysis

Conditions for both columns:

Column:
As listed
Dimension:
As listed
Injection:
Split 0.8:1 @ 150 °C, 1 mL
Carrier Gas:
Helium @ 80 cm/sec (constant flow)
Oven Program:
40 °C (Isothermal)
Detector:
FID @ 250 °C
Sample:
Analytes 0.025 % and internal standards
0.100 % in water
1. Methanol
2. Acetaldehyde
3. Ethanol
4. Isopropanol
5. Acetone
6. t-Butanol (IS)
7. n-Propanol (IS)
8. 2-Butanol (IS)


Comparative separations may not be representative of all applications.
Conditions for both columns:

Column:
As listed
Dimension:
As listed
Part No.:
7HK-G021-36 (ZB-BAC-1)
7HK-G022-32 (ZB-BAC-2)
Injection:
Split 5:1 @ 150 °C, 1 mL
Carrier Gas:
Helium @ 80 cm/sec (constant flow)
Oven Program:
40 °C (Isothermal)
Detector:
FID @ 220 °C
Sample:
Analytes and internal standards
0.100 % in water
1. Methanol
2. Acetaldehyde
3. Ethanol
4. Isopropanol
5. Acetone
6. t-Butanol (IS)
7. n-Propanol (IS)
8. 2-Butanol (IS)

In contrast, the Phenomenex Zebron ZB-BAC-1 & and ZB-BAC-2 columns were able to fully resolve both t-Butanol and 2-Butanol from other target analytes, while maintaining rapid analysis times. Quantitative analysis on the Zebron BAC pair showed equivalent performance between all three internal standards (n-Propanol, t-Butanol, and 2-Butanol). The LOD & and LOQ for this column set were far below the control limits required by current testing regulations.

Estimated Limits of Detection (LOD) and Quantitation (LOQ) on Zebron ZB-BAC1 and ZB-BAC2 phases. The LODs and LOQs are also displayed in units of ppm in addition to % blood alcohol content.

Compound

Zebron ZB-BAC1

Zebron ZB-BAC2

LOD

LOQ

LOD

LOQ

Methanol

0.0003 %
(2.9 ppm)

0.001 %
(9.7 ppm)

0.0003 %
(3.0 ppm)

0.001 %
(10.1 ppm)

Acetaldehyde

0.00002 %
(0.2 ppm)

0.00008 %
(0.8 ppm)

0.00002 %
(0.2 ppm)

0.00006 %
(0.6 ppm)

Ethanol

0.0003 %
(2.6 ppm)

0.0009 %
(8.8 ppm)

0.0002 %
(2.2 ppm)

0.0007 %
(7.4 ppm)

Isopropanol

0.0002 %
(1.8 ppm)

0.0006 %
(5.8 ppm)

0.0001 %
(1.4 ppm)

0.0005 %
(4.6 ppm)

Acetone

0.00008 %
(0.8 ppm)

0.0003 %
(2.6 ppm)

0.00005 %
(0.5 ppm)

0.0002 %
(1.7 ppm)

High reproducibility of Zebron ZB-BAC1 and ZB-BAC2 for blood alcohols. Relative RSD values for replicate injections (n=5) were taken at the lowest concentration in the calibration curve of 0.025 %.


Zebron ZB-BAC-1

Compound
Peak Area % RSD (Absolute)
Peak Area % RSD (Relative to t-Butanol)
Peak Area % RSD (Relative to n-Propanol)
Peak Area % RSD (Relative to 2-Butanol)
Methanol
3.1
2.8
2.7
2.6
Acetaldehyde
2.1
1.6
1.5
1.4
Ethanol
3.4
2.8
2.7
2.6
Isopropanol
3.2
2.6
2.5
2.4
Acetone
2.2
1.6
1.5
1.4

Zebron ZB-BAC-2

Compound
Peak Area % RSD (Absolute)
Peak Area % RSD (Relative to t-Butanol)
Peak Area % RSD (Relative to n-Propanol)
Peak Area % RSD (Relative to 2-Butanol)
Methanol
2.1
1.6
1.4
1.3
Acetaldehyde
3.1
2.7
2.5
2.4
Ethanol
3.2
2.7
2.5
2.4
Isopropanol
2.2
1.6
1.4
1.3
Acetone
3.2
2.7
2.5
2.4