Product Information
Phase Detail
Strata-XL-CW SPE Products
Strata-X-L-CW solid phase extraction (SPE) sorbent is a polymeric large particle weak cation exchange (WCX) phase SPE sorbent for viscous samples
Strata-XL-CW SPE Products
Large Particle Weak Cation Mixed-Mode Solid Phase Extraction (SPE)

A weak cation-exchange functionalized polymeric sorbent that allows for complete retention of basic compounds with a pKa greater than 8, including quaternary amines, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound. The large particle format is ideal for processing large volumes or viscous samples.

Brand
Strata-X
Recommended Use
Extraction of basic compounds, particularly strong bases, present in viscous samples
3 Mechanisms of Retention

Weak Cation-Exchange


π-π Bonding


Hydrophobic Interaction

Available Formats
Strata 96 Well Plate
96-Well Plates
1, 3, and 6 mL Tubes
Giga™ Tubes (12-150 mL Tubes)
Starting Method

For Bases with pKa > 8

Column:
1 mL Methanol
Equilibrate:
1 mL Water, pH 6-7
Load:
Diluted Sample pH 6-7
Wash 1:
1 mL Water, pH 6-7
Wash 2:
1 mL Methanol (collect this fraction to analyze Neutrals/Acids)
Elute Any Acids:
2x 500 µL 5 % Formic Acid in Methanol
Elute Weak Acids:
2x 500 µL 5 % NH4OH in Methanol

Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.