Product Information
Phase Detail
Strata-X-Drug B and Strata-X-Drug B SPE Products
Strata-X-Drug-B solid phase extraction (SPE) sorbent is a polymeric specialty phase that can be used to cleanup basic drugs of abuse
Strata-X-Drug B and Strata-X-Drug B SPE Products
Quick and In-Well Hydrolysis Solid Phase Extraction (SPE)

Proprietary sorbent that is designed and quality controlled for basic drugs of abuse analysis. This sorbent does not require a conditioning/equilibration step to save time and help improve throughput. With Strata-X-Drug B Plus, the hydrolysis step is performed in the well, providing even more time savings and reducing transfer steps.

  • QC Tested for Drug of Abuse
  • No Conditioning or Equilibration Steps Required

Brand
Strata-X
Recommended Use
Extraction of basic drugs from biological samples
General Extraction
  • Extract over 11 drugs using the same sorbent
  • Consolidate methods; 3 methods is all you’ll need to extract 6 different drug classes
  • Time and solvent savings; no conditioning required
  • Consistent, sensitive, and high recoveries of SAMHSA regulated drugs
Retention Mechanism

Strata-X Drug B Protocol

 

Strata-X Drug B Plus Protocol

Starting Method

General Urine Pre-treatment Recommendation

  1. Combine urine sample with appropriate buffer.
  2. Add β-Glucuronidase enzyme according to manufacturer’s recommendation (If using Strata-X-Drug B Plus, these steps can be performed in-well).
  3. Incubate for specified amount of time (If using Strata-X-Drug B Plus,     pulse vacuum to load solution onto sorbent).
  4. Follow appropriate SPE protocol based on analytes of interest.

Choose Your Analytes

  Opiates, 6-MAM, PCP, Amphetamines, Propoxyphene, Healthcare Opiates,
and Methadone*
Marijuana Metabolites Cocaine Metabolites
Condition Not Required Not Required Not Required
Load Pre-treated  urine sample Pre-treated  urine sample Pre-treated  urine sample
Wash 1 600 µL of 100 mM Sodium acetate buffer (pH 5.0) 600 µL of 100 mM Sodium acetate buffer (pH 5.0) 600 µL of 0.1 N Hydrochloric acid
Wash 2 600 µL Methanol 600 µL of Acetonitrile/100 mM Sodium acetate buffer (pH 5.0) (30:70) 600 µL Methanol
Dry 10 minutes under full vacuum 15 minutes under full vacuum 10 minutes under full vacuum
Elute 2x 300 µL of Ethyl acetate/Isopropanol/
Ammonium hydroxide (70:20:10)
2x 300 µL of Ethyl acetate/Isopropanol
(85:15)
2x 300 µL of Ethyl acetate/Isopropanol/
Ammonium hydroxide (70:20:10)



* Opiates, 6-MAM, PCP, Amphetamines, Propoxyphene, Healthcare Opiates, and Methadone can be extracted simultaneously or separately using the same SPE methodology. Methods are written for 30 mg/well Strata-X-Drug B 96-well plate; however they can be scaled to accommodate smaller or larger sample sizes and sorbent masses.