Product Information
Phase Detail
Strata-X-A SPE Products
Strata-X-A solid phase extraction (SPE) sorbent is a polymeric strong anion exchange sorbent that can be used to cleanup acidic compounds
Strata-X-A SPE Products
Strong Anion Mixed-Mode Solid Phase Extraction (SPE)

A strong anion-exchange functionalized polymeric sorbent that allows for complete retention of weakly acidic compounds with a pKa greater than 2, making 100% organic wash conditions possible. A 100% organic wash ensures that the maximum amount of interferences are removed from the target compound.

Brand
Strata-X
Recommended Use
Extraction of basic drugs from biological samples
3 Mechanism of Retention

Strong Anion-Exchange


π-π Bonding


Hydrophobic Interaction


Available Formats
96-Well Plates
Microelution 96-Well Plates
1, 3, and 6 mL Tubes
Giga™ Tubes (12-150 mL Tubes)
Starting Method

For Acids with pKa > 2

Column:
1 mL Methanol
Equilibrate:
1 mL Water, pH 6-7
Load:
Diluted Sample pH 6-7
Wash 1:
1 mL 25 mM Ammonium Acetate Buffered, pH 6-7
Wash 2:
1 mL Methanol (collect this fraction to analyze Neutral/Bases)
Elute Acid:
2x 500 µL 5 % Formic Acid in Methanol

Based on 30 mg/1 mL sorbent mass. The above is a convenient starting point for SPE method development, further optimization may be required to tailor the method for your specific extraction needs.