Product Information
Phase Detail
Rezex ROA-Organic Acid H+ (8%) Ion Exclusion HPLC Columns
Rezex ROA-Organic Acid H+ (8%) Ion Exclusion HPLC Columns is packed with an 8% cross-linked hydrogen ion exclusion media
Rezex ROA-Organic Acid H+ (8%) Ion Exclusion HPLC Columns

  • 8% cross-linked hydrogen
  • For use with organic acids in combination with carbohydrates, alcohols, fatty acids, neutral compounds; amino sugars
  • Uniquely suited to monitor starches, sugars, organic acids, and ethanol in one run for bioethanol fermentation analysis
  • Achieve excellent base line separation between Dp3 and Dp4+

Particle Size
8
Recommended Use
Organic acids alone, organic acids in combination with carbohydrates, alcohols, fatty acids, or neutral compounds, ethanol, acetic acid, glycerol, standard alcohol mixtures, and amino sugars
Cross Reference Guide
Rezex Description Applications Bio-Rad® Aminex® Supelco® Supelcogel™ Waters® Sugar-Pak™ Transgenomic® CARBOSep™ Sepax® Carbomi®
ROA-Organic Acid (USP L22 packing)* 8 % cross-linked HYDROGEN – Organic acids alone
– Organic acids in combination with carbohydrates, alcohols, fatty acids, or neutral compounds
– Ethanol, acetic acid, glycerol, and standard alcohol mixtures
– Amino sugars
HPX-87H
125-0140
Supelcogel
C-610H & H
N/A N/A N/A

Sharper Peak Shape = Easy & Accurate Quantitation

Organic Acids in Wine

Rezex ROA-Organic Acid - Organic Acids in Wine

Columns:
Rezex ROA-Organic Acid Aminex HPX-87H
Dimension:
300 x 7.8 mm
Mobile Phase:
0.005 N H2SO4
Flow Rate:
0.5 mL/min
Temperature:
40 °C
Detection:
UV @ 210 nm
Sample:
1. Citric acid
2. Tartaric acid
3. Succinic acid
4. Lactic acid
5. Acetic acid

Lower Backpressure = Longer Column Lifetimes
and Faster Run Time Capability

Aliphatic Acids

Rezex ROA-Organic Acid - Aliphatic Acids
Aminex and Bio-Rad are registered trademarks of Bio-Rad Laboratories, Inc. Comparative separations may not be representative of all applications.

Columns:
Rezex ROA-Organic Acid Aminex HPX-87H
Dimension:
300 x 7.8 mm
Mobile Phase:
0.005 N H2SO4
Flow Rate:
0.5 mL/min
Temperature:
40 °C
Detection:
UV @ 210 nm
Sample:
1. Oxalic acid
2. Citric acid
3. Tartaric acid
4. Succinic acid
5. Formic acid
6. Acetic acid

Food and Beverage

White Wine

Rezex ROA-Organic Acid - White Wine

Columns:
Rezex ROA-Organic Acid Aminex HPX-87H
Dimension:
300 x 7.8 mm
Part No.:
Mobile Phase:
0.005 N H2SO4
Flow Rate:
0.5 mL/min
Temperature:
40 °C
Detection:
UV @ 210 nm
Sample:
1. Oxalic acid
2. Citric acid
3. Tartaric acid
4. Succinic acid
5. Formic acid
6. Acetic acid

Bioethanol

Rezex ROA-Organic Acid - Bioethanol

Column:
Rezex ROA-Organic Acid
Dimension:
300 x 7.8 mm
Part No.:
Mobile Phase:
0.005 N Sulfuric Acid
Flow Rate:
0.6 mL/min
Temperature:
60 °C
System:
RI @ 40 °C
Detection:
Shimadzu® Prominence® LC-20A System
Sample:
1. Dp4+
2. Dp3
3. Maltose
4. Glucose
5. Lactic Acid
6. Glycerol
7. Acetic Acid
8. Ethanol

Rezex ROA-Organic Acid - Bioethanol

Column:
Rezex ROA-Organic Acid
Dimension:
150 x 7.8 mm
Part No.:
Mobile Phase:
0.005 N Sulfuric Acid
Flow Rate:
0.6 mL/min
Temperature:
60 °C
System:
RI @ 40 °C
Detection:
Shimadzu® Prominence® LC-20A System
Sample:
1. Dp4+
2. Dp3
3. Maltose
4. Glucose
5. Lactic Acid
6. Glycerol
7. Acetic Acid
8. Ethanol

Saccharides 

Monosaccharides with Organic Acids 

Rezex ROA-Organic Acid - Monosaccharides

Column:
Rezex ROA-Organic Acid
Dimension:
300 x 7.8 mm
Part No.:
Mobile Phase:
0.1 % formic acid
Flow Rate:
0.5 mL/min
Temperature:
75 °C
System:
ELSD
Sample:
1. Citric acid
2. Tartaric acid
3. Glucose
4. Fructose
5. Succinic acid

Alcohols

Carboxylic Acids

Rezex ROA-Organic Acid - Carboxylic Acids

Column:
Rezex ROA-Organic Acid
Dimension:
300 x 7.8 mm
Part No.:
Mobile Phase:
Water + 0.5 % Trifluoroacetic Acid
Flow Rate:
1.0 mL/min
Temperature:
40 °C
System:
RI
Sample:
1. Acetylene Carboxylic Acid
2. Maleic Acid
3. Succinic Acid
4. Fumaric Acid

Monosaccharides with Organic Acids 

Rezex ROA-Organic Acid - Monosaccharides with Organic Acids

Column:
Rezex ROA-Organic Acid
Dimension:
300 x 7.8 mm
Part No.:
Mobile Phase:
1 % Phosphoric Acid
Flow Rate:
0.6 mL/min
Temperature:
Ambient
System:
RI
Sample:
1. Glucose
2. N-Acetylglucose
3. N-Acetylgalactosamine

Applications (partial list)

Call us for assistance in selecting the right column for your needs!

Compound App ID No.
Acetic Acid 5515
Acetylene Carboxylic Acid 5517
Ethanol 5512
Formic Acid 5512
Fumaric Acid 5517
Glucose 5514
Isopropanol 5512
Maleic Acid 5517

Compound App ID No.
Methanol 5512
n-Butanol 5512
n-Propanol 5512
n-Acetylgalactosamine 5514
n-Acetylglucose 5514
Oxalic Acid 5515
sec-Butanol 5512
Succinic Acid 5515

Specifications and Operating Recommendations
ROA Organic Acid
Part No. 00H-0138-K0
Ionic Form Hydrogen
Standard Dimensions 300 x 7.8 mm
Matrix Sulfonated Styrene Divinyl Benzene
Cross Linking 8 %
Particle Size (μm) 8
Min. Efficiency (p/m) based on last peak 50,000 (Acetic Acid)
Typical Pressure (psi @ Testing Flow Rate) 580
Max. Pressure (psi @ Max Flow Rate) 1,000
Max. Flow Rate (mL/min) 1.0
Max. Temperature (°C) 85
Typical Mobile Phase 0.005 N H2SO4
pH Range 1-8
Guard Column Part 03B-0138-K0

Cleaning, Regeneration and Storage
Organic Modifiers (Max) 5 % Methanol, IPA, EtOH
Inorganic Modifiers 5 % HNO3, H3PO4
Avoid Acids, Bases, Salts or Metal Ions, pH > 3, > 30 % Acetonitrile
Cleaning Solvent 100 % Water
Flow Rate(mL/min) 0.4
Temperature (°C) 85
Duration (hrs) 12
Regeneration Solvent 0.025 M H2SO4
Flow Rate (mL/min) 0.2
Duration (hrs) 4-16
Ship/Storage Solvent 0.005 N H2SO4