Product Information
Phase Detail
Rezex RCM-Monosaccharide Ca<sup>+2</sup> (8%) Ion Exclusion HPLC Columns<br class="t-last-br" />
Rezex RCM-Monosaccharide Ca+2 (8%) Ion Exclusion Columns are packed with an 8% cross-linked calcium ion exclusion media
Rezex RCM-Monosaccharide Ca+2 (8%) Ion Exclusion HPLC Columns

  • 8% cross-linked calcium
  • For use with monosaccharides and sugar alcohols, including sorbitol and mannitol from sweeteners and corn and cane sugars
  • Class separation of di-, tri-, and tetra- saccharides
  • Easy regeneration with calcium nitrate solutions

Particle Size
8
Recommended Use
Monosaccharides, sugar alcohols, and class separation of di-, tri-, and tetra- saccharides
Cross Reference Guide
Phase Description Applications Bio-Rad® Aminex® Supelco® Supelcogel™ Waters® Sugar-Pak™ Transgenomic® CARBOSep™ Sepax® Carbomi®
RCM-Monosaccharide (USP L19 packing)* 8 % cross-linked CALCIUM – Monosaccharides and sugar alcohols, including sorbitol and mannitol from sweeteners and corn and cane sugars
– Class separation of di-, tri-, and tetra- saccharides
HPX-87C
125-0095
Supelcogel Ca Sugar-Pak 1 CARBOSep CHO-820 Carbomix Ca

Sharper Peak Shape = Easy & Accurate Quantitation

Saccharides

Rezex RCM-Monosaccharide Chromatogram
Comparative separations may not be representative of all applications.

Columns:
Rezex RCM-Monosaccharide
Aminex HPX-87C
Dimension:
300 x 7.8 mm
Mobile Phase:
Water
Flow Rate:
0.6 mL/min
Temperature:
80 °C
Detection:
ELSD
Sample:
1. Melezitose
2. Maltose
3. Glucose
4. Mannose
5. Fructose
6. Ribitol

Orange Juice

Rezex RCM-Monosaccharide Chromatogram
Comparative separations may not be representative of all applications.

Columns:
Rezex RCM-Monosaccharide
Aminex HPX-87C
Dimension:
300 x 7.8 mm
Mobile Phase:
Water
Flow Rate:
0.6 mL/min
Temperature:
80 °C
Detection:
ELSD
Sample:
1. Sucrose
2. Glucose
3. Fructose

Higher Efficiency = Baseline Separation of
Critical Sample Components

Sugars

Rezex RCM-Monosaccharide Chromatogram
Comparative separations may not be representative of all applications.

Columns:
Rezex RCM-Monosaccharide
Bio-Rad® Aminex® HPX-87C
Dimension:
300 x 7.8 mm
Mobile Phase:
Water
Flow Rate:
0.6 mL/min
Temperature:
80 °C
Detection:
ELSD
Sample:
1. Sucrose
2. Glucose
3. Galactose
4. Fructose
5. Sorbitol


Food and Beverage

Apple Juice

Column:
Rezex RCM-Monosaccharide
Dimension:
300 x 7.8 mm
Part No.:
00H-0131-K0
Mobile Phase:
Water
Flow Rate:
0.6 mL/min
Temperature:
75 °C
Detection:
RI
Sample:
1. Sucrose
2. Glucose
3. Fructose

Honey

Column:
Rezex RCM-Monosaccharide
Dimension:
300 x 7.8 mm
Part No.:
00H-0130-K0
Mobile Phase:
Water
Flow Rate:
0.6 mL/min

Temperature:
75 °C
Detection:
RI
Sample:
1. Dp3
2. Dp2
3. Glucose
4. Fructose


Fermentation Broth


Rezex RCM-Monosaccharide Chromatogram

Column:
Rezex RCM-Monosaccharide
Dimension:
300 x 7.8 mm
Part No.:
00H-0130-K0
Mobile Phase:
Water
Flow Rate:
0.5 mL/min
Temperature:
80 °C
Detection:
ELSD
Sample:
1. Sucrose
2. Glucose
3. Galactose
4. Fructose


Saccharides
Column:
Rezex RCM-Monosaccharide
Dimension:
300 x 7.8 mm
Part No.:
00H-0130-K0
Mobile Phase:
Water
Flow Rate:
0.6 mL/min
Temperature:
85 °C
Detection:
RI
Sample:
1. Melezitose
2. Maltose
3. Glucose
4. Mannose
5. Fructose
6. Ribitol
Other Applications

Food Softeners

Rezex RCM-Monosaccharide Chromatogram

Column:
Rezex RCM-Monosaccharide
Dimension:
300 x 7.8 mm
Part No.:
00H-0130-K0
Mobile Phase:
Water
Flow Rate:
0.5 mL/min
Temperature:
60 °C
Detection:
RI
Sample:
1. Glycerol
2. Methoxypolyethylene Glycol
3. Triethylene Glycol
4. Sorbitol
5. Urea


Applications (partial list)

Call us for assistance in selecting the right column for your needs!

Compound App ID No.
Fructose 5504, 5505, 14975, 14981,
14977, 14987
Galactose 14977, 14987
Glucose 5504, 5505, 14975, 14977,
14979, 14981, 14987, 14983
Glycerol 5518
Maltose 5504, 14975
Mannose 5504, 14975
Melezitose 5504, 14975

Compound App ID No.
Methoxypolyethylene Glycol 5518
Ribitol 5504, 14975
Sorbitol 5518, 14977
Sucrose 5505, 14981, 14977, 14987
Tartaric Acid 5504
Triethylene Glycol 5518
Urea 5518

Specifications and Operating Recommendations

Rezex RCM-Monosaccharide
Part No. 00H-0130-K0
Ionic Form Calcium
Standard Dimensions 300 x 7.8 mm
Matrix Sulfonated Styrene Divinyl Benzene
Cross Linking 8 %
Particle Size (μm) 8
Min. Efficiency (p/m) based on last peak 35,000
Typical Pressure (psi @ Testing Flow Rate) 260
Max. Pressure (psi @ Max Flow Rate) 1,000
Max. Flow Rate (mL/min) 1.0
Max. Temperature (°C) 85
Typical Mobile Phase Water
pH Range Neutral
Guard Column Part 03B-0130-K0

Cleaning, Regeneration, and Storage  
Organic Modifiers (Max) 5 % Methanol, IPA, EtOH
Inorganic Modifiers 5 % CaSO4, Ca(NO3)2, CaCl2
Avoid Acids, Bases, Non-Calcium Salts or Metal Ions, > 30 % Acetonitrile
Cleaning Solvent 100 % Water
Flow Rate(mL/min) 0.4
Temperature (°C) 85
Duration (hrs) 12
Regeneration Solvent 0.1 M Ca(NO3)2
Flow Rate (mL/min) 0.2
Duration (hrs) 4-16
Ship/Storage Solvent Water