Product Information
Phase Detail
Phenogel 100 Å GPC/SEC Columns
Phenogel 100 Å GPC Columns can be used with small organics that have a molecular weight range of 500 - 6 K

Phenogel 100 Å GPC/SEC Columns

The Optimal Choice for Aqueous and Mixed Organic Solutions

  • Molecular Weight Range: 500 - 6 K
  • Recommended for use with small organics
  • Wide range of solvent compatibility: packed in tetrahydrofuran (THF); additional shipping solvents (i.e. DMF, Toluene, Chloroform, and others) available on request
  • Temperature stable to 140 °C
  • Reduce solvent consumption and disposal costs up to 65 % while retaining elution profile with Narrow Bore columns
  • Recommended column for USP L21

Applications on Phenogel Fixed Pore 100Å Non-Aqueous
GPC/SEC Columns

Polyethylene Glycol 330

Columns:
Phenogel 5 µm 50 Å, 100 Å, 500 Å
Dimension:
300 x 7.8 mm
Part No.:
00H-0441-K0 (50 Å)
00H-0442-K0 (100 Å)
00H-0443-K0 (500 Å)
Solvent:
THF
Flow Rate:
1.0 mL/min
Temperature:
Ambient
Detection:
Differential Refractometer
Injection Volume:
100 µL 0.25% w/v
Sample:
1. dp7
2. dp6
3. dp5
4. dp4
5. dp3
6. dp2
7. dp1
 
546 MW
458 MW
370 MW
282 MW
194 MW
106 MW
62 MW

Closely Related Hydrocarbons

Columns:
Phenogel 5 µm 50 Å, 100 Å, 500 Å
Dimension:
300 x 7.8 mm
Part No.:
00H-0441-K0 (50 Å)
00H-0442-K0 (100 Å)
00H-0443-K0 (500 Å)
Solvent:
THF
Flow Rate:
1.0 mL/min
Temperature:
Ambient
Detection:
Differential Refractometer
Injection Volume:
100 µL 0.25% w/v
Sample:
1. C40
2. C32
3. C24
4. C20
5. C16
6. C13
 
562 MW
450 MW
338 MW
482 MW
226 MW
684 MW

Isoprenes from In Vitro Translation on Products

The columns were used in tandem to characterize isoprene chain lengths removed from labeled in vitro translation products or cell proteins. The isoprenoids were removed by treatment with Raney nickel and extracted into pentane. The pentane extractable material was hydrogenated over platinum catalyst and injected onto the column. Fractions were collected at 0.5 minute intervals and radioactivity was monitored by liquid scintillation. These saturated hydrocarbon chains were characterized by comparing radioactive peaks to standard retention times.

Chromatogram courtesy of W.Maltese and R.Erdman, Weis Center for Research, Geisinger Clinic.

Columns:
Phenogel 5 µm 50 Å, 100 Å in series
Dimension:
300 x 7.8 mm
Part No.:
00H-0441-K0 (50 Å)
00H-0442-K0 (100 Å)
Solvent:
THF
Flow Rate:
0.25 mL/min
Temperature:
Ambient
Detection:
Differential Refractometer
Sample:
1. Squalene C30
(2, 6, 10, 15, 19, 23-Hexamethyltetracosane)
2. Phytane C20
(2, 6, 10, 14-Tetramethylhexadane)
3. Farnesne C15
(2, 6, 10-Trimethyldodecane)

Environmental and Biomonitoring

An application of preparative GPC columns for the automated purification of environmental samples in concordance to the 3640A EPA method. Results shown are the analysis and purification of PAHs by Phenogel GPC columns and the removal of biomaterial matrix interferences that are known to complicate the analysis of PAHs in biological tissues. This method showed that Phenogel 5 µm 100 Å preparative GPC columns efficiently removed lipids from salmon and mussel samples with high recovery of the target 49 PAHs while demonstrating good repeatability and that it could easily be automated for overnight injection.

Columns:
Phenogel 5 μm 100 Å
Dimension:
300 x 21.2 mm
Part No.:
00H-0442-P0
Mobile Phase:
Methylene chloride
Flow Rate:
5 mL/min
Detector:
Agilent® 1100

Oligomer Composition of Over-the-Counter
Omega-3 Fish Oil Capsules

Among the variety of over-the-counter (OTC) vitamin and nutraceutical products sold to consumers, fish oils containing omega-3 fatty acids are some of the most widely endorsed by healthcare professionals. Three different OTC fish oil supplements were analyzed by GPC chromatography to determine oligomer composition.

One of the three samples analyzed had an oligomer content that would not have met the acceptance criteria of the Ph. Eur. monograph. Further analysis would need to be performed to characterize the exact composition of omega-3 fatty acids in sample OTC-1.

Columns:
Phenogel 5 µm 50 Å, 100 Å, 500 Å
Dimension:
Part No.:
00H-0441-K0 (50 Å)
00H-0442-K0 (100 Å)
00H-0443-K0 (500 Å)
Solvent:
THF
Flow Rate:
0.8 mL/min
Injection Volume:
40 µL
Detector:
RI (35° C)
Temperature:
30° C