Product Information
Phase Detail
Lux i-Amylose-3 Chiral LC Columns
Lux i-Amylose-3 Chiral LC Columns offer greater enantiomeric selectivity
Lux i-Amylose-3 Chiral LC Columns
Your New First Choice Chiral Column!

The immobilization and bonding technology used within the Lux i-Amylose-3 promotes column stability in strong organic solvents, which affords you the ability to expand your chiral separation success with more solvent systems and separation modes.

  • Wide Solvent Flexibility
  • Broad Enantioselectivity
  • Strong Solvent Compatibility

Separation Mode
Chiral
Recommended Use
Lux.RecommendedUse
Achieve Greater Chiral Separation Success

The Lux i-Amylose-3, like the rest of the Lux product line, is an incredibly versatile phase that has been manufactured and developed to give you your desired results again and again. This is especially true under harsh conditions where coated phases are unable to perform.

Lower pressure, all purpose particle size for analytical LC (HPLC) or SFC methods and especially for high performance preparative LC or SFC purification work.


Dependability and Seamless Scalability
Our highest standards of quality will ensure that you are fully satisfied with each and every Lux chiral column as consistent quantitation and results come with every Lux batch and column that we manufacture. With matching selectivity , the 3 μm and 5 μm i-Amylose-3 particle sizes allow you to scale down to increase resolution or easily scale up for preparative purification work.

Conditions for all batches:

Column:
Lux 5 µm i-Amylose-3
Lux 3 µm i-Amylose-3
Dimension:
250 x 4.6 mm
Mobile Phase:
Methanol with 0.05 % Formic Acid
Flow Rate:
0.6 mL/min
Injection Volume:
10 µL (2 mg/mL)
Detection:
UV @ 220 nm
Sample:
1. Carprofen
2. Carprofen

Guaranteed Alternative to CHIRALPAK® IG®

Conditions for both columns:

Column:
Lux 5 µm i-Amylose-3
CHIRALPAK IG
Dimension:
250 x 4.6 mm
Mobile Phase:
Methanol with 0.05 % Formic Acid
Flow Rate:
0.6 mL/min
Injection Volume:
10 µL (2 mg/mL)
Detection:
UV @ 254 nm
Sample:
1. Warfarin
2. Warfarin

Immobilized Selectivity Comparison
Lux immobilized chiral columns offer a wide and complementary range of enantioselectivity for chiral separation projects under normal phase, reversed phase, polar ionic, or SFC separation modes. Below are examples of chiral screening using i-Amylose-3, i-Cellulose-5, and i-Amylose-1 under a variety of screening conditions.

Reversed Phase

Conditions for all columns:

Column:
Lux 5 µm i-Amylose-3
Lux 5 µm i-Cellulose-5
Lux 5 µm i-Amylose-1
Dimension:
250 x 4.6 mm
Part No.:
00G-4779-E0
00G-4756-E0
00G-4762-E0
Mobile Phase:
Water with 0.1 % Diethylamine/
Acetonitrile (35:65)
Flow Rate:
1.0 mL/min
Injection Volume:
10 µL (2 mg/mL)
Detection:
UV @ 254 nm
Sample:
1. Diniconazole


Polar Organic

Conditions for all columns:

Column:
Lux 5 µm i-Amylose-3
Lux 5 µm i-Cellulose-5
Lux 5 µm i-Amylose-1
Dimension:
250 x 4.6 mm
Part No.:
00G-4779-E0
00G-4756-E0
00G-4762-E0
Mobile Phase:
Acetonitrile with 0.1 % Diethylamine
Flow Rate:
1.0 mL/min
Injection Volume:
10 µL (2 mg/mL)
Detection:
UV @ 254 nm
Sample:
1. Thalidomide

Polar Ionic

Conditions for all columns:

Column:
Lux 5 µm i-Amylose-3
Lux 5 µm i-Cellulose-5
Lux 5 µm i-Amylose-1
Dimension:
250 x 4.6 mm
Part No.:
00G-4779-E0
00G-4756-E0
00G-4762-E0
Mobile Phase:
Acetonitrile with 0.1 % Formic Acid
Flow Rate:
1.0 mL/min
Injection Volume:
10 µL (2 mg/mL)
Detection:
UV @ 254 nm
Sample:
1. Indoprofen

If you are using one of the DAICEL columns below: Guaranteed
alternative:
Phase description:
CHIRALPAK® IA® Lux i-Amylose-1 Amylose tris(3,5-dimethylphenylcarbamate)
CHIRALPAK IG® and IG-3 Lux i-Amylose-3 Amylose tris(3-chloro-5-methylphenylcarbamate)
CHIRALPAK IC® and IC-3 Lux i-Cellulose-5 Cellulose tris(3,5-dichlorophenylcarbamate)
CHIRALPAK AD®, AD-H®, AD-3, AD-RH®, and AD-3R Lux Amylose-1 Amylose tris(3,5-dimethylphenylcarbamate)
CHIRALPAK AY®, AY-H®, AY-3, AY-RH, and AY-3R Lux Amylose-2 Amylose tris(5-chloro-2-methylphenylcarbamate)
CHIRALCEL® OD®, OD-H®, OD-3, OD-RH®, and OD-3R Lux Cellulose-1 Cellulose tris(3,5-dimethylphenylcarbamate)
CHIRALCEL OZ, OZ-H®, OZ-3, OZ-RH, and OZ-3R Lux Cellulose-2 Cellulose tris(3-chloro-4-methylphenylcarbamate)
CHIRALCEL OJ®, OJ-H®, OJ-3, OJ-RH®, and OJ-3R Lux Cellulose-3 Cellulose tris(4-methylbenzoate)
CHIRALCEL OX-H, OX-3, OX-RH, and OX-3R Lux Cellulose-4 Cellulose tris(3-chloro-4-methylphenylcarbamate)