Product Information
Phase Detail
Luna PFP(2) HPLC Columns
Luna PFP(2) HPLC columns are packed with a pentafluorophenyl phase that has polar selectivity for cationic compounds
Luna PFP(2) HPLC Columns

The Luna® brand of columns and media is more than just a product line from Phenomenex. It is a pledge to provide you with the highest level of satisfaction for your chromatographic goals. The Luna PFP(2) is a pentafluorophenyl phase with strongly electronegative fluorine groups to provide polar selectivity for cationic compounds, while the rigid bonded phase is a good steric selector.

  • Extremely discerning for halogenated‚ aromatic and conjugated compounds
  • Provides orthogonal selectivity even using traditional reversed phase mobile phase systems
  • PFP Ultra pure, metal-free silica (99.99 % purity) (USP: L43)/li>

Long Column Lifetimes and Excellent Performance
Ultra-pure, metal-free silica (99.99 % purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.

Superior Particle Smoothness

Agilent Technologies® ZORBAX® 5 µm SB-C18
Waters® Symmetry® 5 µm C18
Phenomenex Luna 5 µm C18
4 Mechanisms of Interaction

Luna® PFP(2) columns provide unique selectivity for highly polar compounds, complex natural products, isomers and other closely related compounds. This is achieved by using a propyl linked pentafluorophenyl, which provides multiple retention mechanisms unique to typical reversed phase medias.

  1. Hydrogen Bonding
  2. Dipole-Dipole Interactions
  3. Aromatic and π-π Interactions
  4. Hydrophobic

A typical alkyl phase (C18‚ C8) achieves selectivity through only 1 mechanism of interaction.


PFP Particle
Isomeric Compounds

Positional Isomers of Methylacetophenone

Positional changes on an analyte of interest may effect the compound’s dipole moment. This change can be readily seen by the way the highly electronegative fluorine (F) atoms and other retention mechanisms of the Luna PFP(2) are able to separate positional isomers.
The high efficiencies and bonded phase surface coverage provide for sharp peaks The C 8 columns are generally better hydrogen bond acceptors, and better for acidic compounds.

Luna 3 µm PFP(2)

16298_16299
Conditions same for both columns:

Column:
Luna 3  µm PFP(2)
Luna 3 µm C18(2)
Dimensions
150 x 4.6  mm
Part No.:
Mobile Phase:
Water/Methanol (50:50)
Flow Rate:
1 mL/min
Temperature:
22 °C
Detection:
UV @ 254 nm
Sample:
1. o-Methylacetophenone
2. m-Methylacetophenone
3. p-Methylacetophenone

Aromatic Compounds

Catechins

Aromatic compounds show unique retention characteristics on Luna PFP(2) compared to traditional reversed phase columns. The presence of the aromatic benzene ring in Luna PFP(2) increases the relative attraction between the stationary phase and aromatic analytes, leading to increased retention for these types of compounds.

Luna 3 µm PFP(2)   

App 16294_16295
Conditions same for both columns:

Column:
Luna 3  µm PFP(2)
Luna 3 µm C18(2)
Dimensions
150 x 4.6  mm
Part No.:
Mobile Phase:
A: 0.1 % Formic acid in Water
B: 0.1  % Formic acid in Acetonitrile
Gradient:
A/B (80:20) to (55:45) in 10 min
Flow Rate:
1 mL/min
Temperature:
22 °C
Detection:
UV @ 280 nm
Sample:
1. Gallic acid
2. Epigallo catechin
3. Catechin
4. Epicatechin
5. Epigallocatechin gallate
6. Gallocatechin gallate
7. Epicatechin gallate
8. Catechin gallate