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Product Information
Phase Detail
Luna HILIC HPLC Columns
Luna HILIC HPLC column has a diol phase that provides separation for polar compounds under HILIC conditions

Luna HILIC HPLC Columns

The Luna® brand of columns and media is more than just a product line from Phenomenex. It is a pledge to provide you with the highest level of satisfaction for your chromatographic goals. The Luna HILIC is a diol based silica phase that allows for an denser aqueous layer for increase polar selectivity.

  • Reproducible‚ robust HILIC separations
  • Made for retention of polar compounds
  • Increase mass spectrometry sensitivity

Carbon Load
5.7
Surface Area (m²/g)
200*
Particle Size
3, 5
Recommended Use
Diol phase useful for hydrophilic interaction chromatography mode

Long Column Lifetimes and Excellent Performance

Ultra-pure, metal-free silica (99.99 (= 99.99 %) purity) is the backbone of all Luna material. The resulting high quality particles have a surface smoothness, pore structure, and pore consistency to ensure a more uniform particle shape and greater reproducibility.

Superior Particle Smoothness

Agilent Technologies® ZORBAX®
Waters® Symmetry®
Phenomenex Luna

Increase MS Sensitivity and Retention for Polar Compounds

Hydrophilic Interaction Liquid Chromatography (HILIC) is a separation mode where the partitioning of polar solutes from the high concentration‚ water-miscible‚ organic mobile phase into the hydrophilic surface environment creates separations. Polar solutes exhibit increased retention and elute in the order of increasing hydrophilicity.

Water Soluble Vitamins

Conditions:

Column:
Luna 5 μm HILIC
Dimension:
150 x 4.6 mm
Mobile Phase:
Acetonitrile/100 mM
Ammonium Formate, pH 3.2 (90:10)
Flow Rate:
1.0  mL/min
Temperature:
Ambient
Detection:
UV @ 260 nm
Sample:
1. PABA
2. Nicotinamide
3. Riboflavin
4. Nicotinic Acid
5. Pyridocine
6. Thiamine