Luna Omega PS C18 HPLC Columns: Phenomenex

Improve Peak Shape for Bases Without the Headache

Luna Omega PS C18 is a unique mixed interaction stationary phase that provides incredibly useful polar and non-polar retention. The surface of the PS C18 contains a positive charge which aids in the retention of acidic compounds through ionic interactions, while the C18 ligand promotes general reversed phase retention. This mixed-mode selectivity allows for greater separation between compounds with varying functional groups.

Compatible with All Analytical HPLC/UHPLC Systems*
Fully Scalable Particle Size UHPLC HPLC Preparative
Charged Surface C18 that is 100 % Aqueous Stable

Scalable Particle Sizes

*Luna Omega analytical columns are compatible with all LC system with standard LC column end fittings threaded in 10-32. Please contact us at Phenomenex.com/chat for any questions and information about fitting compatibility and any other column internal diameters then analytical.

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A C18 With a Positive Twist

Luna Omega PS C18 has been fine tuned and manufactured by Phenomenex to provide a mixed selectivity that is highly useful for method development involving either combinations of polar and nonpolar compounds, or just one single compound class with small changes in functional groups.

Enhanced Selectivity and Polar Retention

With its positive surface (PS) the Luna Omega PS C18 provides valuable increase in retention of acids through ionic/polar interactions. In addition, the C18 ligand also stimulates hydrophobic retention that greatly promotes increased resolution between analytes of interest.

MMA and Succinic Acid

Conditions for all columns:

Columns: Luna Omega 1.6 μm PS C18 ACE Excel 2 μm C18-AR
Dimension: 50 x 2.1 mm
Mobile Phase: A: Water with 0.1 % Formic Acid B: Acetonitrile with 0.1 % Formic Acid
Gradient: Time (min) 0 5 5.1 7	% B 0 50 0 0
Flow Rate: 0.5 mL/min
Temperature: 22 °C
Detection: MS/MS (SCIEX® API 4000™)
Samples: 1. Succinic acid 2. MMA

Think Outside the Traditional C18 Selectivity

Traditional C18 phases may not always be the best option. A HPLC and UHPLC column that has both polar and hydrophobic versatility allows for great method development flexibility.

Acids/Bases/Neutrals

Conditions for all columns:

Columns: Luna Omega 1.6 μm PS C18 ACQUITY BEH 1.7 μm C18
Dimension: 50 x 2.1 mm
Mobile Phase: A: Water with 0.1 % Formic Acid B: Acetonitrile with 0.1 % Formic Acid
Gradient: Time (min) 0 5 5.01 8	% B 5 95 5 5
Flow Rate: 0.4 mL/min
Temperature: 22 °C
Detection: UV @ 254 nm
Samples: 1. Uracil 2. Pindolol 3. Chlorpheniramine 4. Nortriptyline 5. 3-Methyl-4-nitrobenzoic acid 6. 5-Methyl salicylaldehyde 7. Hexanophenone

Better Peak Shape for Bases

While traditional alkyl phases are prone to show tailing for basic compounds because of secondary interactions occurring at the silica surface, the surface of the Luna Omega PS C18 was designed with positive charges that serve to repel strong basic species and consistently display sharp peak shape.

Acids/Bases/Neutrals

Conditions for all columns:

Columns: Luna Omega 1.6 μm PS C18 Thermo Scientific^® Hypersil GOLD 1.9 μm C18 Agilent^® ZORBAX 1.8 µm SB-C18
Dimension: 50 x 2.1 mm
Mobile Phase: A: Water with 0.1% Formic Acid B: Acetonitrile with 0.1% Formic Acid
Gradient: Time (min) 0 3 3.1 5	% B 3 80 3 3
Flow Rate: 0.5 mL/min
Temperature: 22 °C
Detection: MS/MS (SCIEX API 4000)
Samples: Insulin