Luna Omega HPLC/UHPLC LC Columns
World Renowned HPLC Media Reinvented for UHPLC!
The cutting edge thermally modified Luna® Omega particle delivers high efficiency, ruggedness, reproducibility, and dependability for a wide range of chromatographic analyses. Luna Omega HPLC and UHPLC columns are built upon more then 20 years of applied knowledge, invention, and customer experience in the manufacture and packing of LC columns. The particle’s novel design and proprietary manufacturing process results in greater particle inertness, a stronger particle morphology, and more consistent porosity.
- Greater separation muscle and retention
- Consistent porous structure and elimination of micropores
- Extreme ruggedness and reproducibility
Luna Omega Silica
One of the world’s leading HPLC brands, now enhanced for incredible HPLC and UHPLC performance! Luna Omega 1.6, 3, and 5 µm columns culminate 20 years of technological prowess, advancements, and innovation from Phenomenex! With astounding efficiency levels, highly versatile selectivities, and trusted accuracy, Luna Omega columns will take your chromatographic experience to a new level.
Thermal Modified Pore Structure
Controlled Column Reproducibility
Batch-to-batch and column-to-column, Luna® Omega media and columns are designed to be consistent and incredibly accurate tools for your analysis. Each batch and column are quality tested to ensure dependability and reproducibility.
Column: |
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Dimensions: 50 x 2.1 mm |
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Part No.: |
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Mobile Phase: A: Water B: Acetonitrile |
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Gradient:
Time (min)
0
6
6.01
8 |
% B
20
60
20
20 |
Flow Rate: 0.4 mL/min |
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Temperature: Ambient |
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Detection: UV @ 220 nm |
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Scalable from UHPLC to HPLC to PREP
Luna Omega C18
Luna Omega C18 is an excellent first choice for chromatographers who are just starting method development or attempting to improve upon existing chromatographic results with other C18s. With its higher performance potential, excellent retention profile, and greater inertness, the Luna Omega C18 was designed to be the new all-purpose UHPLC solution for industries all over the world.
Material Characteristics
| Phase |
Particle Sizes (µm) |
Pore Size (Å) |
Surface Area (m2/g) |
Carbon Load (%) |
pH Stability |
Pressure Limit (Bar) |
USP Column Classification |
|---|---|---|---|---|---|---|---|
| C18 | 1.6 | 100 | 200 | 11 | 1.5 – 8.5* | 1034 | L1 |
UHPLC Efficiency Comparison
Conditions for all columns:
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Dimension: 50 x 2.1 mm
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Mobile Phase: Acetonitrile/Water (65:35)
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Flow Rate: 0.5 mL/min
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Temperature: Ambient
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Comparative separations may not be representative of all applications.
*Efficiency (plates/meter) comparison is based on peak performance associated with the compound naphthalene for all columns.
Detection: UV @ 254 nm |
System: ACQUITY UPLC®
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Sample: Naphthalene*
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Luna Omega PS C18
Luna Omega PS C18 is a unique mixed-mode stationary phase that provides incredibly useful polar and non-polar retention. The surface of the PS C18 contains a positive charge which aids in the retention of acidic compounds through ionic interactions, while the C18 ligand promotes general reversed phase retention. This mixed-mode selectivity allows for greater separation between compounds with varying functional groups.
Organic Acids
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Column:
Luna Omega 1.6 μm PS C18
ACQUITY HSS 1.8 μm T3 |
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Dimensions:
100 x 2.1 mm
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Mobile Phase:
A: Water with 0.1% Formic Acid
B: Acetonitrile with 0.1% Formic Acid
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Gradient:
Time (min)
0
5
5.1
7
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% B
0
50
0
0
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Flow Rate:
0.5 mL/min
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Temperature:
22 °C
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Detection:
MS/MS (SCIEX API 4000)
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Sample:
1. Lactic acid
2. Succinic acid 3. MMA 4. Glutaric acid 5. Ethylmalonic acid 6. Methyl succinate 7. Hippuric acid 8. Suberic acid 9. Sebacic acid |
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Material Characteristics
| Phase |
Particle Sizes (µm) |
Pore Size (Å) |
Surface Area (m2/g) |
Carbon Load (%) |
pH Stability |
Pressure Limit (Bar) |
USP Column Classification |
|---|---|---|---|---|---|---|---|
| PS C18 | 1.6, 3, 5 | 100 | 260 | 9 | 1.5 – 8.5* | 1034/345** | L1 |
*pH stability under gradient conditions. pH stability is 1.5-10 under isocratic conditions.
**1.6 µm Luna Omega columns are pressure rated up to 1034 bar and 3 or 5µm are stable up to 345 bar.
Luna Omega Polar C18
Luna Omega Polar C18 is a novel UHPLC stationary phase capable of providing a unique selectivity within a wide elution window and increased retention for both polar and non-polar analytes. The all-purpose C18 ligand provides hydrophobic interactions while a polar modified particle surface provides enhanced polar retention and also aqueous stability. These attributes make the Luna Omega Polar C18 an excellent choice for balanced retention of polar and hydrophobic compounds as well as to solely enhance retention of highly polar compounds.
Material Characteristics
| Phase |
Particle Sizes (µm) |
Pore Size (Å) |
Surface Area (m2/g) |
Carbon Load (%) |
pH Stability |
Pressure Limit (Bar) |
USP Column Classification |
|---|---|---|---|---|---|---|---|
| Polar C18 | 1.6, 3, 5 | 100 | 260 | 9 | 1.5 – 8.5* | 1034/345** | L1 |
*pH stability under gradient conditions. pH stability is 1.5-10 under isocratic conditions.
**1.6 µm Luna Omega columns are pressure rated up to 1034 bar and 3 or 5µm are stable up to 345 bar.
SAMHSA Workplace Drug Testing Screen
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Column:
Luna Omega 1.6 µm Polar C18
ACQUITY HSS 1.8 µm T3 |
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Dimensions:
50 x 2.1 mm
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Mobile Phase:
A: Water with 0.1 % Formic Acid
B: Acetonitrile with 0.1 % Formic Acid
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Gradient:
Time (min)
0
2
3
3.1
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% B
15
100
100
15
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Flow Rate:
0.4 mL/min
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Temperature:
25 °C
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Detection:
MS/MS (SCIEX API 4000)
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Sample:
1. Morphine
2. Codeine 3. Amphetamine 4. MDA 5. Methamphetamine 6. 6-MAM 7. MDMA 8. MDEA 9. BZE 10. PCP 11. THC-COOH 12. THC |
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Alternative C18 Selectivity Method Development Flexibility
Pharmaceutical Drug Impurity Profile
Column: Luna Omega 1.6 μm PS C18 Luna Omega 1.6 μm Polar C18 Luna Omega 1.6 μm C18 |
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| Dimensions:
50 x 2.1 mm
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Mobile Phase: A: Water with 0.1 % Formic Acid
B: Acetonitrile with 0.1 % Formic Acid |
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| Gradient:
Time (min)
0
10
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% B
5
40
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| Flow Rate:
0.4 mL/min
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Temperature:
22 °C
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| Detection:
UV @ 254 nm
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| Sample:
Proprietary drug impurity profile
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Luna Omega SUGAR
Luna Omega SUGAR breaks ground as it combines the performance benefits of thermally modified fully porous particles with a novel HILIC stationary phase that excels at polar compound retention and selectivity.
- Improved carbohydrate retention and separation with multi-functional selectivity that contains amide/amino stationary phase and polar endcapping
- Enhanced lifetime with highly robust and efficient thermally modified fully porous particle
- QC tested for sugars to ensure reliable quality
Material Characteristics
| Phase |
Particle Sizes (µm) |
Pore Size (Å) |
Surface Area (m2/g) |
Carbon Load (%) |
pH Stability |
Pressure Limit (Bar) |
USP Column Classification |
|---|---|---|---|---|---|---|---|
| Luna Omega SUGAR | 3 | 100 | 260 | <2 | 2.0-7.0 | 345 |
L8 |
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Column:
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Dimensions:
150 x 4.6 mm
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Mobile Phase:
Acetonitrile/Water (75:15)
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Flow Rate:
1 mL/min
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Detection:
RI (Refractive Index)
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Sample:
1. Fructose
2. Glucose 3. Galactose 4. Sucrose 5. Maltose 6. Lactose |
