Luna Omega HPLC/UHPLC LC Columns

World Renowned HPLC Media Reinvented for UHPLC!

The cutting edge thermally modified Luna® Omega particle delivers high efficiency, ruggedness, reproducibility, and dependability for a wide range of chromatographic analyses. Luna Omega HPLC and UHPLC columns are built upon more then 20 years of applied knowledge, invention, and customer experience in the manufacture and packing of LC columns. The particle’s novel design and proprietary manufacturing process results in greater particle inertness, a stronger particle morphology, and more consistent porosity.

Greater separation muscle and retention
Consistent porous structure and elimination of micropores
Extreme ruggedness and reproducibility

How It Works
Product Finder
Phases
Documents
Webinars
APPLICATIONS
FAQs
Order

Luna Omega Silica

One of the world’s leading HPLC brands, now enhanced for incredible HPLC and UHPLC performance! Luna Omega 1.6, 3, and 5 µm columns culminate 20 years of technological prowess, advancements, and innovation from Phenomenex! With astounding efficiency levels, highly versatile selectivities, and trusted accuracy, Luna Omega columns will take your chromatographic experience to a new level.

Thermal Modified Pore Structure

Controlled Column Reproducibility

Batch-to-batch and column-to-column, Luna® Omega media and columns are designed to be consistent and incredibly accurate tools for your analysis. Each batch and column are quality tested to ensure dependability and reproducibility.

Conditions for all columns:

Column: Luna Omega 1.6 µm C18
Dimensions: 50 x 2.1 mm
Part No.: 00B-4742-AN
Mobile Phase: A: Water B: Acetonitrile
Gradient: Time (min) 0 6 6.01 8	% B 20 60 20 20
Flow Rate: 0.4 mL/min
Temperature: Ambient
Detection: UV @ 220 nm

Scalable from UHPLC to HPLC to PREP

Comparative separations may not be representative of all applications.

Luna Omega C18

Luna Omega C18 is an excellent first choice for chromatographers who are just starting method development or attempting to improve upon existing chromatographic results with other C18s. With its higher performance potential, excellent retention profile, and greater inertness, the Luna Omega C18 was designed to be the new all-purpose UHPLC solution for industries all over the world.

Material Characteristics

Phase	Particle Sizes (µm)	Pore Size (Å)	Surface Area (m²/g)	Carbon Load (%)	pH Stability	Pressure Limit (Bar)	USP Column Classification
C18	1.6	100	200	11	1.5 – 8.5*	1034	L1

UHPLC Efficiency Comparison

Conditions for all columns:

Dimension: 50 x 2.1 mm
Mobile Phase: Acetonitrile/Water (65:35)
Flow Rate: 0.5 mL/min
Temperature: Ambient

Comparative separations may not be representative of all applications.
*Efficiency (plates/meter) comparison is based on peak performance associated with the compound naphthalene for all columns.

Detection: UV @ 254 nm
System: ACQUITY UPLC®
Sample: Naphthalene*

Luna Omega PS C18

Luna Omega PS C18 is a unique mixed-mode stationary phase that provides incredibly useful polar and non-polar retention. The surface of the PS C18 contains a positive charge which aids in the retention of acidic compounds and better peak shape for bases through ionic interactions, while the C18 ligand promotes general reversed phase retention. This mixed-mode selectivity allows for greater separation between compounds with varying functional groups.

Organic Acids

Conditions for all columns:

Column: Luna Omega 1.6 μm PS C18 ACQUITY HSS 1.8 μm T3
Dimensions: 100 x 2.1 mm
Mobile Phase: A: Water with 0.1% Formic Acid B: Acetonitrile with 0.1% Formic Acid
Gradient: Time (min) 0 5 5.1 7	% B 0 50 0 0
Flow Rate: 0.5 mL/min
Temperature: 22 °C
Detection: MS/MS (SCIEX API 4000)
Sample: 1. Lactic acid 2. Succinic acid 3. MMA 4. Glutaric acid 5. Ethylmalonic acid 6. Methyl succinate 7. Hippuric acid 8. Suberic acid 9. Sebacic acid

Material Characteristics

Phase	Particle Sizes (µm)	Pore Size (Å)	Surface Area (m²/g)	Carbon Load (%)	pH Stability	Pressure Limit (Bar)	USP Column Classification
PS C18	1.6, 3, 5	100	260	9	1.5 – 8.5*	1034/345**	L1

*pH stability under gradient conditions. pH stability is 1.5-10 under isocratic conditions.
**1.6 µm Luna Omega columns are pressure rated up to 1034 bar and 3 or 5µm are stable up to 345 bar.

Luna Omega Polar C18

Luna Omega Polar C18 is a novel UHPLC stationary phase capable of providing a unique selectivity within a wide elution window and increased retention for both polar and non-polar analytes. The all-purpose C18 ligand provides hydrophobic interactions while a polar modified particle surface provides enhanced polar retention and also aqueous stability. These attributes make the Luna Omega Polar C18 an excellent choice for balanced retention of polar and hydrophobic compounds as well as to solely enhance retention of highly polar compounds.

Material Characteristics

Phase	Particle Sizes (µm)	Pore Size (Å)	Surface Area (m²/g)	Carbon Load (%)	pH Stability	Pressure Limit (Bar)	USP Column Classification
Polar C18	1.6, 3, 5	100	260	9	1.5 – 8.5*	1034/345**	L1

*pH stability under gradient conditions. pH stability is 1.5-10 under isocratic conditions.
**1.6 µm Luna Omega columns are pressure rated up to 1034 bar and 3 or 5µm are stable up to 345 bar.

SAMHSA Workplace Drug Testing Screen

Conditions for both columns:

Column: Luna Omega 1.6 µm Polar C18 ACQUITY HSS 1.8 µm T3
Dimensions: 50 x 2.1 mm
Mobile Phase: A: Water with 0.1 % Formic Acid B: Acetonitrile with 0.1 % Formic Acid
Gradient: Time (min) 0 2 3 3.1	% B 15 100 100 15
Flow Rate: 0.4 mL/min
Temperature: 25 °C
Detection: MS/MS (SCIEX API 4000)
Sample: 1. Morphine 2. Codeine 3. Amphetamine 4. MDA 5. Methamphetamine 6. 6-MAM 7. MDMA 8. MDEA 9. BZE 10. PCP 11. THC-COOH 12. THC

Alternative C18 Selectivity Method Development Flexibility

The combination of available Luna® Omega stationary phases represent an outstanding tool set for the separation of acids, bases, neutrals, or mixtures. Use the C18 to focus on hydrophobic interactions or the mixed-mode functionality of the Polar C18 and PS C18 to get enhanced retention of both polar and non-polar compounds.

Pharmaceutical Drug Impurity Profile

Conditions for all columns:

Column: Luna Omega 1.6 μm PS C18 Luna Omega 1.6 μm Polar C18 Luna Omega 1.6 μm C18
Dimensions: 50 x 2.1 mm
Mobile Phase: A: Water with 0.1 % Formic Acid B: Acetonitrile with 0.1 % Formic Acid
Gradient: Time (min) 0 10	% B 5 40
Flow Rate: 0.4 mL/min
Temperature: 22 °C
Detection: UV @ 254 nm
Sample: Proprietary drug impurity profile

Luna Omega SUGAR

Luna Omega SUGAR breaks ground as it combines the performance benefits of thermally modified fully porous particles with a novel HILIC stationary phase that excels at polar compound retention and selectivity.

Improved carbohydrate retention and separation with multi-functional selectivity that contains amide/amino stationary phase and polar endcapping
Enhanced lifetime with highly robust and efficient thermally modified fully porous particle
QC tested for sugars to ensure reliable quality

Material Characteristics

Phase	Particle Sizes (µm)	Pore Size (Å)	Surface Area (m²/g)	Carbon Load (%)	pH Stability	Pressure Limit (Bar)	USP Column Classification
Luna Omega SUGAR	3	100	260	<2	2.0-7.0	345	L8

Conditions for all columns:

Column: Luna Omega 3 μm SUGAR Fully Porous 3 μm NH₂ Hybrid Fully Porous 3.5 μm Amide
Dimensions: 150 x 4.6 mm
Mobile Phase: Acetonitrile/Water (75:15)
Flow Rate: 1 mL/min
Detection: RI (Refractive Index)
Sample: 1. Fructose 2. Glucose 3. Galactose 4. Sucrose 5. Maltose 6. Lactose