Product Information
Phase Detail
Kinetex F5 Core-Shell HPLC Columns
100% aqueous novel pentafluorophenyl phase (L43) that can be used in reversed phase, HILIC, 2D LC, and SFC

Kinetex F5 Core-Shell HPLC Columns

Maximize Retention Interactions and Performance

Combine core-shell performance, multiple retention mechanisms and the Kinetex® F5 column’s ability to be run in a variety of separation modes (reversed phase, HILIC, SFC, 2D LC, and 100% aqueous) and you now have an impeccable method development tool at your disposal.

  • Compatible with All Analytical HPLC/UHPLC Systems*
  • 100 % Aqueous Mobile Phases Stable
  • 5 Mechanisms of Retention and Selectivity
  • USP: L43

*Kinetex analytical columns are compatible with all LC system with standard LC column end-fittings threaded in 10-32. Please contact us at Phenomenex.com/chat for any questions and information about fitting compatibility and any other column internal diameters.


Particle Size
1.6, 2.6, 5
Recommended Use
Highly reproducible pentafluorophenylpropyl phase, exceptional for halogenated, conjugated, isomeric, or highly polar compounds.

F5 - A Novel Selectivity For Tough Separations

This pentafluorophenyl propyl column provides a very high degree of steric selectivity to separate structural isomers. The electronegative fluorine groups offer high selectivity for cationic compounds.

Dependable F5 Selectivity

While older pentafluorophenyl phases (PFP, PFPP, F5, etc.) are based on existing bonding techniques and technologies that promote irreproducibility. The Kinetex F5 was meticulously designed by Phenomenex R&D and its customers, to provide consistently accurate and high performance results.



Conditions same for both samples:
Columns:
Kinetex 2.6 µm F5
Ascentis Express 2.7 µm F5
Dimension:
50 x 4.6 mm
Mobile Phase:
A: Water with 0.1% Formic Acid
B: Acetonitrile with 0.1% Formic Acid
Isocratic:
5-95 % B over 5 minutes
Flow Rate:
1.85 mL/min
Temperature:
Ambient
Detection:
UV @ 254 nm
Sample:
1. Uracil
2. Pindolol
3. Chlorpheniramine
4. Nortriptyline
5. 3-Methyl-4-Nitrobenzoic acid
6. 5-Methyl Salicyl Aldehyde
7. Hexaphenone

Take Advantage of Multiple Interactive Mechanisms

The multiple interactive mechanisms of the Kinetex F5 (pentafluorophenyl ) column successfully separate methoxybenzene isomers, while the Kinetex C18 column, which has minimal bonding interactions, cannot separate the methoxybenzene isomers. This demonstrates that columns that rely primarily on hydrophobic interactions may not be the first choice for the separation of isomeric compounds and a column with multiple interactive mechanisms may be required.

Conditions for all columns:

Columns:
Kinetex 2.6 µm F5
Kinetex 2.6 µm C18
Dimension:
150 x 4.6 mm
Mobile Phase:
A: Water with 0.1 % TFA (Trifluoroacetic acid)
B: Acetonitrile
Isocratic:
A/B (65:35)
Flow Rate:
1 mL/min
Temperature:
Ambient
Detection:
UV @ 254 nm
Sample:
1. 1,2,3-Trimethoxybenzene
2. 1,2-Dimethoxybenzene
3. 1,2,4-Trimethoxybenzene
4. 1,4-Dimethoxybenzene
5. Methoxybenzene
6. 1,3-Dimethoxybenzene