The EZ:faast amino acid analysis procedure consists of a solid phase extraction step followed by a derivatization and a liquid/liquid extraction; derivatized samples are quickly analyzed by gas chromatography-mass spectrometry. The solid phase extraction is performed via a sorbent packed tip that binds amino acids while allowing interfering compounds to flow through. Amino acids on sorbent are then extruded into the sample vial and quickly derivatized with reagent at room temperature in aqueous solution. Derivatized amino acids concomitantly migrate to the organic layer for additional separation from interfering compounds. Organic layer is then removed, evaporated, and re-suspended in re-dissolution solvent and analyzed on a GC-MS system. Total sample preparation time takes around 8 minutes and analysis is performed in
around 7 minutes for a total start-to-finish time of around 15 minutes.