Product Information
Phase Detail
Free (Physiological) Amino Acid Analysis by GC-FID or GC-NPD
Ezfasst Free (Physiological) Amino Acid Analysis by GC-FID or GC-NPD Kits allow for simple analysis of physiological amino acids

Free (Physiological) Amino Acid Analysis by GC-FID or GC-NPD

The EZ:faast amino acid analysis procedure consists of a solid phase extraction step followed by a derivatization and a liquid/liquid extraction; derivatized samples are quickly analyzed by gas chromatography with FID or NPD detection. The solid phase extraction is performed via a sorbent packed tip that binds amino acids while allowing interfering compounds to flow through. Amino acids on sorbent are then extruded into the sample vial and quickly derivatized with reagent at room temperature in aqueous solution. Derivatized amino acids concomitantly migrate to the organic layer for additional separation from interfering compounds. An aliquot from the organic layer is analyzed on a GC instrument with either a FID or NPD detector. Total sample preparation time takes around 8 minutes and analysis is performed in around 7 minutes for a total start-to-finish time of around 15 minutes.