EZ:faast kits provide reagents and supplies for sample preparation derivatization and GC-FID, GC-NPD, GC-MS, and LC-MS analysis of over 60 amino acids and di-peptides in complex matrices and protein hydrolysates.
Additionally the EZ:faast method offers:
Step 1 – 0 min
Pipette sample and combine internal standard (Yellow)
Step 2 – 1 min
Pipette sample through SPE sorbent tip
Advantage: No lengthy protein or urea removal
Step 3 – 2 min
Draw wash solution (blue) through SPE sorbent tip
Advantage: Outstanding sample purity for less contamination
Step 4 – 3 min
Expel amino acids (blue) with SPE sorbent from tip with Eluting Medium (clear)
Advantage: Almost no loss of amino acids
Step 5 – 4 min
Add organic derivatizing reagent (orange)
Advantage: Derivatization is complete in 1 min and can be done in aqueous phase
Step 6 – 6 min
Extract amino acid derivatives from aqueous layer (light green) into organic layer (orange)
Advantage: Additional purification step
Step 7 – 7 min
Sample preparation complete inject onto GC-FID, GC-NPD, or GC-MS system
Advantage: Very stable derivatization sample reduces losses due to degradation
Step 8 – 15 min
Analyze the chromatogram
Advantage: Full resolution of over 50 amino acids and related compounds in a 7 minute GC run