Product Information
Brand
Clarity SPE
Clarity sample preparation products provide effective purification for oligonucleotides and available in desalting cartridges as well as reversed phase and ion-exchange selectivities.
Clarity SPE
The Clarity BioSolutions sample preparation portfolio is composed of unique solid phase extraction chemistries packed in 96-well plate and cartridge formats designed to efficiently and effectively purify oligonucleotides.
- Works for desalting and clean up of biological samples
- Specially formulated buffer solutions available for optimal performance
- Quick protocols for rapid LC-MS/MS results
- No liquid-liquid extractions (LLE)
Oligonucleotide Purification Products
Clarity OTXâ„¢ Extraction Kits
Prior to LC-MS analysis, therapeutic oligonucleotides must be isolated from interferences such as salts, sugars, large proteins, and genomic DNA. Using a mixed-mode solid phase extraction (SPE) sorbent in conjunction with carefully formulated buffers, Clarity OTX consistently delivers high recoveries.
Clarity QSPâ„¢ High-Throughput Purification
Purify trityl-on synthetic DNA or RNA samples in 20 minutes using Clarity QSP 96-well plate and buffer system. Final elution delivers >90 % typical purities/recoveries for in vivo applications and downstream analysis by LC-MS or CE.
Clarity RP-Desaltingâ„¢ Quick Salt Removal
Clarity RP-Desalting tubes utilize reversed phase, in lieu of size exclusion, to eliminate salt and reagent from trityl-off synthetic oligo mixtures in order to capitalize on the speed and loading capacity benefits.
Material Characteristics
| Clarity Products | Particle Support |
Bonded Phase |
Particle shape/Size (µm) |
Pore Size (Ã…) |
Surface Area (m2/g) |
|---|---|---|---|---|---|
| Clarity QSPâ„¢ Cartridges | Polymer (PSDVB) | Hydrophilic polymer coating | 35 | 500 | 300 |
| Clarity RP-Desaltingâ„¢ Tubes | Silica | C18 | 55 | 140 | 300 |
| Clarity OTXâ„¢ Extraction Plates |
Polymer (surface modified PSDVB) |
Mixed-mode anion exchanger | 33 | 85 | 800 |
Clarity OTX and Clarity QSP are patented by Phenomenex. U.S. Patent No. 7,119,145
Clarity BioSolutions Product Selection
Purification
| Clarity QSPâ„¢ | Clarity Oligo-RPâ„¢ Clarity Oligo-XT |
Clarity RP-Desaltingâ„¢ | ||
|---|---|---|---|---|
| Primary Use | High-throughput, trityl-on RPC purification |
RP-HPLC purification of failure sequences from target sequences |
Quick removal or salt & excess reagent | |
| Purities | >90% | >90% | ~70% | |
| Recoveries | ~90% | ~70% | ~70% | |
| Synthesis Scale Load | Up to 50 µmol | Up to 50 µmol | Up to 1 µmol | |
| Oligo Types | DNA, RNA/RNAi, Thioates, Dye-labled, Modified | |||
Characterization / Analysis
| Clarity Oligo-RPâ„¢ | Clarity Oligo-MSâ„¢ Clarity Oligo-XT |
Clarity Oligo-OTXâ„¢ | |
|---|---|---|---|
| Primary Use | RP-LC-MS analysis with optimized selectivity and sensitivity |
Rapid, high efficiency RP-LC-MS analysis for QC and characterization |
Extraction of oligo therapeutics from biological samples for LC-MS bioanalysis |
| Oligo Length | ≤ 60 mer | ≤ 60 mer | ≤ 40 mer |
| Recommended Mobile Phase | TEA / HFIP | TEA/HFIP/MeOH | N/A |
Headquarters
411 Madrid Avenue, Torrance, CA 90501
t: (310) 212-0555 f: (310) 328-7768
info@phenomenex.com
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