The Clarity BioSolutions sample preparation portfolio is composed of unique solid phase extraction chemistries packed in 96-well plate and cartridge formats designed to efficiently and effectively purify oligonucleotides.
Clarity Products | Particle Support |
Bonded Phase |
Particle shape/Size (µm) |
Pore Size (Ã…) |
Surface Area (m2/g) |
---|---|---|---|---|---|
Clarity QSPâ„¢ Cartridges | Polymer (PSDVB) | Hydrophilic polymer coating | 35 | 500 | 300 |
Clarity RP-Desaltingâ„¢ Tubes | Silica | C18 | 55 | 140 | 300 |
Clarity OTXâ„¢ Extraction Plates |
Polymer (surface modified PSDVB) |
Mixed-mode anion exchanger | 33 | 85 | 800 |
Clarity OTX and Clarity QSP are patented by Phenomenex. U.S. Patent No. 7,119,145
Clarity QSPâ„¢ | Clarity Oligo-RPâ„¢ Clarity Oligo-XT |
Clarity RP-Desaltingâ„¢ | ||
---|---|---|---|---|
Primary Use | High-throughput, trityl-on RPC purification |
RP-HPLC purification of failure sequences from target sequences |
Quick removal or salt & excess reagent | |
Purities | >90% | >90% | ~70% | |
Recoveries | ~90% | ~70% | ~70% | |
Synthesis Scale Load | Up to 50 µmol | Up to 50 µmol | Up to 1 µmol | |
Oligo Types | DNA, RNA/RNAi, Thioates, Dye-labled, Modified |
Clarity Oligo-RPâ„¢ | Clarity Oligo-MSâ„¢ Clarity Oligo-XT |
Clarity Oligo-OTXâ„¢ | |
---|---|---|---|
Primary Use | RP-LC-MS analysis with optimized selectivity and sensitivity |
Rapid, high efficiency RP-LC-MS analysis for QC and characterization |
Extraction of oligo therapeutics from biological samples for LC-MS bioanalysis |
Oligo Length | ≤ 60 mer | ≤ 60 mer | ≤ 40 mer |
Recommended Mobile Phase | TEA / HFIP | TEA/HFIP/MeOH | N/A |