Product Information
Phase Detail
Clarity Oligo-XT
Clarity Oligo-XT HPLC Columns is a core-shell HPLC column used for purification of oligonucleotides.
Clarity Oligo-XT
A Sensitive Solution for Oligo Characterization and Bioanalysis

Unlike traditional fully porous oligo columns, Clarity Oligo-XT relies on the power of core-shell technology to provide extremely high efficiencies for both low and high oligo concentrations. Because the Clarity Oligo-XT particle is not fully porous, analytes spend less time diffusing into and out of the pores as they travel through the column, resulting in less band broadening for higher peak efficiencies, making Clarity Oligo-XT a great choice for analyses that require sensitivity such as oligo characterization and oligo analysis from bioanalytical samples.

Particle Size
1.7,2.6,5
Recommended Use
Rapid, high efficiency RP-LC-MS from purification to characterization
LC-MS Analysis

Oligo-XT

An increased need for TK and PK/PD studies for oligo therapeutics has lead to an increase in LC-MS/MS analysis because of the specificity, sensitivity, and shorter method development times as compared to ligand binding assays or ELISA. Because sensitivity is of the utmost importance, it is crucial to select an LC column that provides both separation and high efficiencies to ensure accurate results. 

Clarity Core-Shell


Conventional Fully Porous

The Core-Shell Advantage

Unlike traditional fully porous oligo columns, Clarity Oligo-XT relies on the power of core-shell technology to provide extremely high efficiencies for both low and high oligo concentrations. Because the Clarity Oligo-XT particle is not fully porous, analytes spend less time diffusing into and out of the pores as they travel through the column, resulting in less band broadening for higher peak efficiencies.

Fully
Porous
  Clarity
Core-Shell
Average Efficiency
Gain with Clarity*
VS.

90 % Higher

VS.

85 % Higher

VS.

20 % Higher

Clarity Oligo-XT is patented by Phenomenex. U.S. Patent No. 7,563,367 and 8,658,038 and foreign counterparts.
*May not be representative of all applications.

Sensitive, Reliable Analysis

Calibration curve of BNA in mini pig liver homogenate

Application 16021: Quadratic regression 1-1000 ng/mL

Column:
Clarity 5 μm Oligo-XT
Dimension:
50 x 2.1 mm
Part No.:
00B-4745-AN
HPLC System:
Shimadzu® Nexera® X2 UHPLC
Mobile Phase:
A: 1.0 % HFIP & 0.1 % DIEA with 10 μM EDTA in Methanol
B: 1.0 % HFIP & 0.1 % DIEA with 10 μM EDTA in Methanol / Water (50:50 v/v)
Gradient:
Time (min)
0.5
2.5
3.0
3.5
4.0
5.0
 
% B
30
60
100
100
30
30
Flow Rate:
500 μL/min
Temperature:
40 ˚C
Detection:
Thermo Q Exactive™ Hybrid Quadrupole-Orbitrap™
Mass Spectrometer, HESI, negative polarity
Injection Volume:
10 μL