Product Information
Phase Detail
Claricep Spherical C18
Claricep spherical C18 flash columns provide significant performance over traditional flash columns on the market
Claricep Spherical C18

  • Significantly improved performance over regular flash columns
  • Silica deactivated by proprietary process
  • Alternative selectivity for complex purification requirements
claricep_spherical_c18

Better Separation Performance

Tetrandrine Extracted from Natural Products, Formulation

Comparative separations may not be representative of all applications.
Flash Conditions

Column A:
Brand X Flash Irregular C18 (40-60 μm 100 Å, 12 g, 3 columns in tandem)
Column B:
Claricep™ Spherical C18 (20-35 μm 100 Å, 12 g, 3 columns in tandem)
Column C:
Claricep Spherical C18 (20 μm 100 Å, 12 g, 3 columns in tandem)
Mobile Phase:
A: Water B: Methanol
Gradient:
Time (min)
0
100
 
% B
85
85
Flow Rate:
83 mL/min
Detector:
UV @ 254/282 nm
Sample
Tetrandrine

Ink Sample Interference / Irregular vs. Spherical

Take 50 μL of two kinds of raw ink samples separately, filter through with a 0.22 μm Nylon Filter and then load onto individual flash columns.

Flash Conditions

Column A:
Claricep™ Irregular C18 (40-60 μm 60 Å, 20 g, 2 columns in tandem)
Column B:
Claricep™ Spherical C18 (40-60 μm 100 Å, 20 g, 2 columns in tandem)
Mobile Phase:
A: Water B: Methanol
Gradient:
Time (min)
0
60
 
% B
10
100
Flow Rate:
26 mL/min
Detector:
UV@254 nm (red), @ 220 nm (blue)
Instrument:
CHEETAH™ MP 200

Polypeptide Isolation

HPLC Analysis

Column:
LC C18, 5 μm, 100 Å, 4.6 × 150 mm
Mobile Phase:
A: Water + 0.01 % TFA
B: Acetonitrile (A/B, 73:27)
Flow Rate:
1 mL/min
Detector:
UV @ 205 nm
Injection Volume:
1 μL

Flash Purification

Column:
Claricep™ C18 (40-60 μm, 100 Å, 12 g)
Part No.:
SO240012-0
Mobile Phase:
Acetonitrile/Water
Flow Rate:
15 mL/min
Detector:
UV @ 205/280 nm
Sample Volume:
2 mL

Purity Test of the Fraction

CLARICEP Spherical C18 Screw-on Columns

Which Screw-on Series should I order ?

CLARICEP i-Series

The i-Series features a managed column head space with a secured screw-on lid. This new design allows either loading of liquid samples directly onto the column head or loading of impregnated solid sample directly into the space. Users will benefit from:

  • Choice of loading method based on sample properties
  • Narrow band for liquid samples because of wide loading area
  • Dry-loading of solid impregnated samples minimizes band broadening
  • Customized loading method upon user preference

Ordering Information
Part Number starts with “SN”. As an example, to order SO240012-0 in i-series, Part Number to order is SN-SO240012-0

CLARICEP c-Series

The c-Series shares the same design, but the column outlet does not have a luer lock structure, this simplifies tubing connection across various flash systems.

Compatible with instruments from the following:
  • Agela Technologies
  • Buchi
  • Grace
  • Interchim
  • Other brands

Ordering Information
Part Number starts with “C”. As an example, to order SO240012-0 in c-series, Part Number to order is C-SO240012-0

CLARICEP s-Series

The s-Series columns are fully packed without a head void. In addition, the Luer lock fittings for both inlet and outlet allows easy operation of tandem columns or the coupling of a loading cartridge.

Compatible with instruments from the following:
  • Biotage

Ordering Information
Part Number starts with “S”. As an example, to order SO240012-0 in s-series, Part Number to order is S-SO240012-0