Product Information
Phase Detail
bioZen WCX
bioZen WCX HPLC columns consist of non-porous polymeric particles that can separate proteins from their acidic and basic variants
bioZen WCX

bioZen WCX was constructed with linear polycarboxylate chains grafted to monosized non-porous polymeric particles, to sinuously envelop and separate proteins from acidic and basic variants. With a highly tuned and controlled manufacturing process, bioZen WCX affords scientists a way to reproducibly characterize heterogeneity with an added advantage of excellent recovery using bioinert titanium column hardware.

bioZen WCX Ion-Exchange Column

bioZen WCX Monosized Polymeric Non-Porous Particle

Monosized particles grafted with linear polycarboxylate chains to envelop and separate proteins from acidic/basic variants

Minimize your priming and promote better recovery
with our BioTi™ biocompatible hardware
Monosized Particles for Optimal Consistency

Our specially crafted polymeric particle is produced with outstanding precision so that you can be confident in increased performance and minimized variation between lots.

Comparative separations may not be representative of all applications.

Columns:
bioZen 6 µm WCX
Dimension:
250 x 4.6 mm
Part No.:
00G-4777-EO
Mobile Phase:
A: CX-1 Gradient Buffer A (pH 5.6)
B: CX-1 Gradient Buffer B (pH 10.2)
Gradient:
0-100 % B in 20 minutes
Flow Rate:
1 mL/min
Temperature:
30 °C
Detection:
UV @ 280 nm
Sample:
Trastuzumab
Note:
CX-1 Gradient Buffers available from Thermo
Fisher Scientific™
Charge Variant Analysis
bioZen WCX was crafted to consistently decipher between native protein variants that arise from PTMs within a therapeutics creation and development. The linear polycarboxylate chains grafted to monosized non-porous polymeric particles, envelop and separate proteins from acidic and basic protein variants. With such a highly. tuned and controlled manufacturing process, bioZen WCX media affords scientists a way to reproducibly characterize heterogeneity while taking advantage of excellent recovery through high particle inertness and bioinert titanium BioTi™ column hardware.

Trastuzumab (MES Salt Gradient)

Column:
bioZen 6 μm WCX
Dimension:
250 x 4.6 mm
Part No.:
00G-4777-E0
Mobile Phase:
A: 20 mM MES (pH 5.6)
B: 20 mM MES + 300 mM NaCl (pH 5.6)
Gradient:
Time (min)
0
1
31
31.1
34
35
 
% B
15
15
45
100
100
15
Flow Rate:
1 mL/min
Temperature:
30 °C
Detection:
UV @ 280 nm
Sample:
Trastuzumab

Trastuzumab (pH Gradient Buffer)

Column:
bioZen 6 μm WCX
Dimension:
250 x 4.6 mm
Part No.:
00G-4777-E0
Mobile Phase:
A: CX -1 (pH 5.6) pH Gradient Buffer*
B: CX -1 (pH 10.2) pH Gradient Buffer*
Gradient:
Time (min)
0
1
21
23
24
 
% B
0
0
100
100
0
Flow Rate:
1 mL/min
Temperature:
30 °C
Detection:
UV @ 280 nm
Sample:
Trastuzumab

* From Thermo Fisher Scientific® Inc.

Acidic and Basic Variant Analysis

Whether using a pH gradient or salt gradient, the bioZen 6μm WCX consistently outperforms ProPac™ WCX-10 columns in charge variant analysis.

Comparative separations may not be representative of all applications.

Columns:
bioZen 6 µm WCX
ProPac 10 µm WCX-10
Dimension:
250 x 4.6 mm (bioZen)
250 x 4 mm (ProPac)
Mobile Phase:
A: CX-1 Gradient Buffer A (pH 5.6)
B: CX-1 Gradient Buffer B (pH 10.2)
Gradient:
0-100 % B in 20 minutes
Flow Rate:
1.0 mL/min (bioZen)
0.8 mL/min (ProPac)
Temperature:
30 °C
Detection:
UV @ 280 nm
Sample:
As indicated
Note:
CX-1 Gradient Buffers available from Thermo Fisher Scientific™
Native Mode Mass Spectrometry with bioZen WCX
Ion-exchange chromatography (IEX) is a technique employed in the characterization of biotherapeutics under nondenaturing conditions. Standard mobile phase conditions for these techniques are high in salt concentration, rendering them incompatible with mass spectrometry (MS) detection. Native MS represents a cutting edge approach to employ MS compatible mobile phases for non-denaturing IEX analysis. Observe and measure the non-covalent interactions and charge variants of proteins at high resolution using bioZen WCX.

Charge Variant Analysis of Trastuzumab using a bioZen 6 µm WCX
Column with a pH Gradient and Native MS Detection

Columns:
bioZen 6 μm WCX
Dimension:
50 x 2.1 mm
Part No.:
00B 4777 AN
Mobile Phase:
A: 20 mM Ammonium Acetate, pH 5.2
B: 5 mM Ammonium Acetate, pH 10.2
Gradient:
20-50 % in 5 min
Flow Rate:
0.5 mL/min
Temperature:
30 °C
Detection:
SCIEX® X500B Q-TOF
Sample:
Trastuzumab, 50 µg

MS TIC Chromatogram on a 250 x 2.1mm bioZen 6 μm WCX
using pH Gradient Buffer System

LC-MS/MS Conditions

Columns:
bioZen 6 μm WCX
Dimension:
50 x 2.1 mm
Part No.:
00B 4777 AN
Mobile Phase:
A: 20 mM Ammonium Acetate, pH 5.2
B: 5 mM Ammonium Acetate, pH 10.2
Gradient:
20-50 % in 5 min
Flow Rate:
0.5 mL/min
Temperature:
30 °C
Detection:
SCIEX® X500B Q-TOF
Sample:
Trastuzumab, 50 µg