Product Information
Phase Detail
bioZen SEC-3
bioZen Intact XB-C8 core-shell UHPLC columns provide extremely for impurity profiling and characterization of intact biologic fragments
bioZen SEC-3

High inertness and particle consistency of both bioZen™ SEC columns drives greater reproducibility from injection-to-injection. Combine this with the bioinert BioTi hardware and good aggregate recovery is no longer something that you’re missing out on.

bioZen SEC columns provide:
  • High efficiency on both HPLC and UHPLC
  • Extreme inertness and robustness
  • Finely tuned high and low MS separation windows


Particle Size
1.8
Recommended Use
High molecular weight (HMW) separation range of 10 k – 700 kDa
Inside bioZen BioTi Hardware

Conditions for both columns:

Columns:
Dimension:
150 x 4.6 mm
Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM Sodium Sulfate, pH 5.0
Flow Rate:
0.3 mL/min
Temperature:
Ambient
Detection:
UV @ 280 nm
Sample:
1. γ-Globulin, 5 mg/mL
2. Ovalbumin, 1 mg/mL

Aggregate Analysis by Size Exclusion

Conditions same for all samples:

Column:
bioZen 1.8 µm SEC-3
Dimension:
300 x 4.6 mm
Part No.:
00H-4772-E0
Mobile Phase:
50 mM Potassium Phosphate + 
250 mM Potassium Chloride (pH 6.8)
Flow Rate:
3.5 mL/min
Temperature:
30 °C
Detection:
UV @ 280 nm
Sample:
As noted

Conditions same for all samples:

Column:
bioZen 1.8 µm SEC-3
Dimension:
300 x 4.6 mm
Part No.:
00H-4772-E0
Mobile Phase:
50 mM Potassium Phosphate + 
250 mM Potassium Chloride (pH 6.8)
Flow Rate:
3.5 mL/min
Temperature:
30 °C
Detection:
UV @ 280 nm
Sample:
As noted

Conditions same for all samples:

Column:
bioZen 1.8 µm SEC-3
Dimension:
300 x 4.6 mm
Part No.:
00H-4772-E0
Mobile Phase:
50 mM Potassium Phosphate + 
250 mM Potassium Chloride (pH 6.8)
Flow Rate:
3.5 mL/min
Temperature:
30 °C
Detection:
UV @ 280 nm
Sample:
As noted