Product Information
Phase Detail
BioZen Peptide-XB-C18
BioZen Peptide-XB-C18

Digested mAbs or ADCs typically include a large body of compounds which are crucial to understanding post translation modifications. So we designed our bioZen™ Peptide columns to offer highly useful and unique retention profiles. bioZen Peptide XB-C18 allows for fast and effective elution windows by utilizing core-shell particles to gain sharper peaks, better peak capacities, and overall higher sensitivity.

bioZen Peptide columns provide:

  • Unique retention profiles
  • Greater peak coverage
  • High efficiency on both HPLC and UHPLC

Particle Size
1.7,2.6
Recommended Use
Overall retention of both acidic and basic peptides for both peptide mapping and peptide quantitation applications
Inside bioZen BioTi Hardware

Conditions for both columns:
Column:
bioZen 1.8 μm SEC-3
Dimension:
150 x 4.6 mm
Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM
Sodium Sulfate, pH 5.0
Flow Rate:
0.3 mL/min
Temperature:
Ambient
Detection:
UV @ 280 nm
Sample:
1. γ-Globulin, 5 mg/mL
2. Ovalbumin, 1 mg/mL
Peptide Mapping: Reversed Phase LC

Cetuximab Peptide Map

Anti Rituximab Fab Peptide Map


Columns:
bioZen 1.6 µm Peptide PS-C18
 bioZen 2.6 µm Peptide XB-C18
Dimension:
150 x 2.1 mm
Part No.:
00F-4770-AN
00F-4768-AN
Mobile Phase:
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile

Gradient:
Time (min)
0
5
60
65
70
75
78
 
% B
1
1
50
50
95
95
95
Flow Rate:
bioZen 1.6 µm Peptide PS-C18: 0.3 mL/min
bioZen 2.6 µm Peptide XB-C18: 0.6 mL/min
Temperature:
40 °C
Detection:
UV @ 214 nm

Conditions for all columns:

Columns:
bioZen 2.6 μm Peptide XB-C18
bioZen 1.6 m Peptide PS-C18
Dimension:
150 x 2.1 mm
Part No.:
00F-4768-AN
00F-4770-AN
Mobile Phase:
A: 0.1% Formic Acid in Water
B: 0.1% Formic Acid in Acetonitrile
Gradient:
Time (min)
0
5
45
 
% B
3
3
48
Flow Rate:
0.5 mL/min
Temperature:
50 °C
Detection:
MS/MS
Samples:
1. As Noted

TICs for Infliximab, XB-C18 vs. PS-C18

LC-UV conditions
Column:
bioZen 2.6 µm Peptide XB-C18
bioZen 1.6 µm Peptide PS-C18
Part No.:
00F-4768-AN (XB-C18)
00F-4770-AN (PS-C18)
Mobile Phase:
A. 0.1 % Formic Acid in Water
B. 0.1 % Formic Acid in Acetonitrile
Gradient:
1-50 %  B in 50 minutes
Flow Rate:
0.3 mL/min
Detection:
SCIEX® X500B Q-TOF
Sample:
Infliximab Digest, 1 µg