Product Information
Phase Detail
bioZen Peptide-PS-C18
Mixed-mode C18 column that provides excellent retention for biologics.
bioZen Peptide-PS-C18

bioZen Peptide PS-C18 is a mixed-mode phase that combines a positively charged surface ligand and a C18 ligand. The result is an HPLC column phase that provides excellent retention for biologics.

Particle Size
1.6,3
Recommended Use
Excellent retention of peptides for both peptide mapping and peptide quantitation applications
Inside bioZen BioTi™ Hardware
Conditions for both columns:
Column:
bioZen 1.8 μm SEC-3
Dimension:
150 x 4.6 mm
Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM Sodium Sulfate, pH 5.0

Flow Rate:
0.3 mL/min
Temperature:
Ambient
Detection:
UV @ 280 nm
Sample:
1. γ-Globulin, 5 mg/mL
2. Ovalbumin, 1 mg/mL
Peptide Mapping: Reversed Phase LC

Cetuximab Peptide Map



Anti Rituximab Fab Peptide Map


Column:
bioZen 1.6 µm Peptide PS-C18
bioZen 2.6 µm Peptide XB-C18
Dimension:
150 x 2.1 mm
Part No.:
00F-4774-AN
00F-4768-AN
Mobile Phase:
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
Gradient:
Time (min)
0
5
60
65
70
75
78
 
% B
1
1
50
50
95
95
95
Flow Rate:
bioZen 1.6 µm Peptide PS-C18: 0.3 mL/min
bioZen 2.6 µm Peptide XB-C18: 0.6 mL/min
Temperature:
40 °C
Detection:
UV @ 214

Conditions for all columns:

Column:
bioZen 2.6 μm Peptide XB-C18
 bioZen 1.6 m Peptide PS-C18
Dimension:
150 x 2.1 mm
Part No.:
00F-4768-AN
00F-4770-AN
Mobile Phase:
A: 0.1% Formic Acid in Water
B: 0.1% Formic Acid in Acetonitrile
Gradient:
Time (min)
0
5
45
 
% B
3
3
48
 
Flow Rate:
0.5 mL/min
Temperature:
50 °C
Detection:
MS/MS
Sample:
1. As Noted

TICs for Infliximab, XB-C18 vs. PS-C18

LC-UV conditions
Column:
bioZen 2.6 µm Peptide XB-C18
bioZen 1.6 µm Peptide PS-C18
Part No.:
00F-4768-AN (XB-C18)
00F-4770-AN (PS-C18)
Mobile Phase:
A. 0.1 % Formic Acid in Water
B. 0.1 % Formic Acid in Acetonitrile
Gradient:
1-50 %  B in 50 minutes
Flow Rate:
0.3 mL/min
Detection:
SCIEX® X500B Q-TOF
Sample:
Infliximab Digest, 1 µg

Comparative separations may not be representative of all applications.
Conditions for all columns:

Column:
bioZen 1.6 µm Peptide PS-C18
Waters ACQUITY 1.7 µm Peptide CSH
Dimension:
150 x 2.1 mm
Part No.:
00F-4770-AN (PS-C18)
Mobile Phase:
A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in Acetonitrile
Gradient:
1-50 % B int 50 minutes
Temperature:
50 °C
Detection:
SCIEX X500B Q-TOF
Sample:
Trastuzumab Digest, 1 µg