Product Information
Phase Detail
bioZen Peptide-PS-C18
Mixed-mode C18 column that provides excellent retention for biologics.
bioZen Peptide-PS-C18

bioZen Peptide PS-C18 is a mixed-mode phase that combines a positively charged surface ligand and a C18 ligand. The result is an HPLC column phase that provides excellent retention for biologics.

Inside bioZen BioTi™ Hardware
Conditions for both columns:
Column:
bioZen 1.8 μm SEC-3
Dimension:
150 x 4.6 mm
Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM Sodium Sulfate, pH 5.0

Flow Rate:
0.3 mL/min
Temperature:
Ambient
Detection:
UV @ 280 nm
Sample:
1. γ-Globulin, 5 mg/mL
2. Ovalbumin, 1 mg/mL
Peptide Mapping: Reversed Phase LC

Cetuximab Peptide Map



Anti Rituximab Fab Peptide Map


Column:
bioZen 1.6 µm Peptide PS-C18
bioZen 2.6 µm Peptide XB-C18
Dimension:
150 x 2.1 mm
Part No.:
00F-4774-AN
00F-4768-AN
Mobile Phase:
A: 0.1 % TFA in Water
B: 0.1 % TFA in Acetonitrile
Gradient:
Time (min)
0
5
60
65
70
75
78
 
% B
1
1
50
50
95
95
95
Flow Rate:
bioZen 1.6 µm Peptide PS-C18: 0.3 mL/min
bioZen 2.6 µm Peptide XB-C18: 0.6 mL/min
Temperature:
40 °C
Detection:
UV @ 214

Conditions for all columns:

Column:
bioZen 2.6 μm Peptide XB-C18
 bioZen 1.6 m Peptide PS-C18
Dimension:
150 x 2.1 mm
Part No.:
00F-4768-AN
00F-4770-AN
Mobile Phase:
A: 0.1% Formic Acid in Water
B: 0.1% Formic Acid in Acetonitrile
Gradient:
Time (min)
0
5
45
 
% B
3
3
48
 
Flow Rate:
0.5 mL/min
Temperature:
50 °C
Detection:
MS/MS
Sample:
1. As Noted

TICs for Infliximab, XB-C18 vs. PS-C18

LC-UV conditions
Column:
bioZen 2.6 µm Peptide XB-C18
bioZen 1.6 µm Peptide PS-C18
Part No.:
00F-4768-AN (XB-C18)
00F-4770-AN (PS-C18)
Mobile Phase:
A. 0.1 % Formic Acid in Water
B. 0.1 % Formic Acid in Acetonitrile
Gradient:
1-50 %  B in 50 minutes
Flow Rate:
0.3 mL/min
Detection:
SCIEX® X500B Q-TOF
Sample:
Infliximab Digest, 1 µg

Comparative separations may not be representative of all applications.
Conditions for all columns:

Column:
bioZen 1.6 µm Peptide PS-C18
Waters ACQUITY 1.7 µm Peptide CSH
Dimension:
150 x 2.1 mm
Part No.:
00F-4770-AN (PS-C18)
Mobile Phase:
A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in Acetonitrile
Gradient:
1-50 % B int 50 minutes
Temperature:
50 °C
Detection:
SCIEX X500B Q-TOF
Sample:
Trastuzumab Digest, 1 µg