Product Information
Phase Detail
bioZen Oligonucleotides
bioZen Oligo HPLC column provides targeted selectivity for oligos
bioZen Oligonucleotides

Digested mAbs or ADCs typically include a large body of compounds which are crucial to understanding post translation modifications. So we designed two bioZen ™ Peptide columns to offer highly useful and unique retention profiles . Each allows for fast and effective elution windows by utilizing either high efficiency core shell or thermally modified fully porous particles to gain sharper peaks, better peak capacities, and overall higher sensitivity .

bioZen Peptide columns provide:

  • Unique retention profiles
  • Greater peak coverage
  • High efficiency on both HPLC and UHPLC

bioZen Oligo Column: Biocompatible Titanium Hardware and Core-shell Technology Enhance Recovery and Performance for Olignoucleotide Analysis

Two Particle Platforms

Thermally Modified Fully Porous

bioZen Peptide PS-C18
1.6 µm and 3 µm
Excellent retention by combined positively
charged surface ligand and C18 ligand.

Core-Shell Technology

bioZen Peptide XB-C18
1.7 µm and 2.6 µm
Overall retention of both acidic and basic peptides through C18 stationary phase with di-isobutyl side chains.


Peptide Mapping: Reversed Phase LC

Trastuzumab Biosimilar Peptide Map

 

Infliximab Biosimilar Peptide Map


Column:
bioZen 1.6 µm Peptide PS-C18
 bioZen 2.6 µm Peptide XB-C18
Dimension:
150 x 2.1 mm
Part No.:
00F-4770-AN
00F-4768-AN
Mobile Phase:
A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in Acetonitrile

Gradient:
Time (min)
0
0.5
50
55
56
 
% B
1
1
50
50
95
Flow Rate:
0.3 mL/min
Temperature:
40 °C
Detection:
QTOF (SCIEX® X500B)

Conditions for all columns:

Column:
bioZen 2.6 μm Peptide XB-C18
 bioZen 1.6 m Peptide PS-C18
Dimension:
150 x 2.1  mm
Part No.:
00F-4768-AN
00F-4770-AN
Mobile Phase:
0.1% TFA in Water
0.1% TFA in Acetonitrile
Gradient:
Time (min)
0
5
160
65
70
75
78
 
% B
1
1
50
50
95
95
95
Flow Rate:
0.3 mL/min
Temperature:
40 °C
Detection:
UV @ 214 nm
Sample:
As noted

Peptide Mapping: LC-MS/MS

Conditions for all columns:

Column:
  bioZen 2.6 µm Peptide XB-C18
  bioZen 1.6 µm Peptide PS-C18
Dimension:
150 x 2.1  mm
Part No.:
00F-4768-AN
00F-4770-AN
Mobile Phase:
A: 0.1% Formic Acid in Water
B: 0.1% Formic Acid in Acetonitrile
Gradient:
Time (min)
0
5
45
 
% B
3
3
48
Flow Rate:
0.5 mL/min
Temperature:
50 °C
Detection:
MS/MS
Sample:
As noted