Product Information
Phase Detail
bioZen Glycan
bioZen Glycan 2.6 µm core-shell HPLC columns provide optimized separation of released and labelled glycans.
bioZen Glycan

The unique selectivity of the bioZen Glycan was designed to provide higher order separations of released and labeled glycans. With a 2.6 μm core-shell particle size, customers using either HPLC or UHPLC systems, can draw upon a high efficiency bioZen Glycan particle run at higher linear velocities, to easily provide sharper peak shapes and faster elution windows, without high UHPLC pressures. Under HILIC-FLR or HILIC-MS conditions, the bioZen Glycan excels with increased polar retention and selectivity

Minimize your priming with our BioTi™ Biocompatible hardware

bioZen Titanium BioTi Hardware

Application ID 24717

Columns:
Dimension:
150 x 4.6 mm
Mobile Phase:
50 mM Dipotassium Phosphate + 100 mM Sodium Sulfate, pH 5.0
Flow Rate:
0.3 mL/min
Temperature:
Ambient
Detection:
UV @ 280 nm
Sample:
1. γ-Globulin, 5 mg/mL
2. Ovalbumin, 1 mg/mL

Conditions for both separations:

Column:
bioZen 2.6 µm Glycan
Dimension:
150 x 2.1 mm
Part No.:
00F-4773-AN
Mobile Phase:
A: 100 mM Ammonium Formate, pH 4.5
B: Acetonitrile
Gradient:
Time (min)
0
10
24
38.5
38.6
40.6
40.7
48
 
% B
78
74.5
72
55.9
40
40
78
78
Flow Rate:
0.5 mL/min
Temperature:
50 °C
Detection:
FLD ex/em 285/345 nm
Samples:
As noted

Sample Preparation Solutions

N-Gycan Clean-Up HILIC Solid Phase Extraction (SPE)

High recovery of labeled, released N-glycans in a microelution 96-well plate format allowing for streamlined processing and clean-up of small sample volumes.


MagBeads Streptavidin Coated

Higher binding capacity magnetic particles result in faster and reliable purification, clean-up, and isolation of proteins and peptide molecules.


bioZen Glycan Batch-to-Batch Reproducibility

Conditions for all separations:

Column:
bioZen 2.6 μm Glycan
Dimension:
100 x 2.1 mm
Part No.:
00D-4773-AN
Mobile Phase:
A: 100 mM Ammonium Formate, pH 4.5
B: Acetonitrile
Gradient:
Time (min)
0
16
25.9
27.2
27.3
30
 
% B
76
72
40
40
76
76
Flow Rate:
0.5 mL/min
Temperature:
50 °C
Detection:
FLD ex/em 330/420 nm
Samples:
Human IgG Glycan Library

2-AB Labeled Bovine Fetuine Sialylated Glycans

Column:
bioZen 2.6 μm Glycan
Dimension:
100 x 2.1 mm
Part No.:
00D-4773-AN
Mobile Phase:
A: 100 mM Ammonium Formate, pH 4.5
B: Acetonitrile
Gradient:
Time (min)
0
20
24
24.1
30
 
% B
60
20
20
60
60
Flow Rate:
0.5 mL/min
Temperature:
80 °C
Detection:
FLD ex/em 330/420 nm
Sample:
Bovine Fetuin

Glycan Analysis: HILIC

Column:
bioZen 2.6 μm Glycan
Dimension:
100 x 2.1 mm
Part No.:
00D-4773-AN
Mobile Phase:
A: 100 mM Ammonium Formate, pH 4.5
B: Acetonitrile
Gradient:
Time (min)
0
16
25.9
27.2
27.3
30
 
% B
76
72
40
40
76
76
Flow Rate:
As Noted
Temperature:
50 °C
Detection:
FLD ex/em 330/420 nm
Sample:
Human IgG Glycan Library