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How do you pretreat a whole blood sample for SPE?
A simple pretreatment is to add 10% TCA (trichloroacetic acid) aqueous solution in the ratio of 1:1.
Centrifuge and collect the supernatant. Another simple way to pretreat whole blood is to perform protein precipitation with cold acetonitrile-methanol (85:15) in the ratio of 1:2. After centrifuging, the supernatant is diluted with solvent appropriate for extraction loading.A more thorough pretreatment is converting whole blood into blood plasma. Centrifuging anti-coagulated blood gives blood plasma as supernatant. Blood plasma preparation kits with prepackaged tubes containing an anticoagulant are commercially available.
For more information on sample pretreatment for extraction using Strata and Strata-X, please contact us.
I have seen you have a number of different applications for Vitamin D, which ones should I consider?
When attempting to analyze vitamin D, it is important to consider the source of the sample.That is, if samples are from a biological source the vitamin D is likely to be present as the 25-hydroxyvitamin D3 . The difficulty will be in separation of 25-hydroxy and 3-epi-25-hydroxy vitamin D3, and we have applications specific for this critical pair.If the separation is vitamin D from a food stuff or supplement, it will be present just as Vitamin D3 (cholecalciferol) and this is a much more straightforward separation. We have applications on this as well as other fat-soluble vitamins.
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