Rapid and Simple Extraction and Analysis of Vitamin D2 and D3 from Dietary Supplements by QuEChERS and HPLC (TN-0094)
Vitamins accounts for a large percentage of all dietary/nutritional supplements purchased and consumed. Vitamin D specifically is a fat-soluble vitamin that has been known for its ability to enhance intestinal absorption of calcium, iron, magnesium, phosphate, and zinc. In order to analyze vitamin D3 the compound must be extracted from the matrix before it can be analyzed using RPHPLC. Presented here is a rapid and simple extraction procedure using QuEChERS salts and HPLC analysis using a Kinetex 2.6 μm Biphenyl Core-shell HPLC column.