Documents
Utilizing Biphasic Salt and pH Gradients for Charge Variant Analysis (TN-1252)
Cation exchange chromatography (CEX) is a favorable analytical method for characterization of proteins as it is non-denaturing and can be used to qualitatively and quantitively identify charge heterogeneity of a protein samples. This technote will describe mobile phase effects on weak cation exchange (WCX) chromatography including a biphasic salt and pH gradient.
Utilizing Biphasic Salt and pH Gradients for Charge Variant Analysis (TN-1252)
Cation exchange chromatography (CEX) is a favorable analytical method for characterization of proteins as it is non-denaturing and can be used to qualitatively and quantitively identify charge heterogeneity of a protein samples. This technote will describe mobile phase effects on weak cation exchange (WCX) chromatography including a biphasic salt and pH gradient.
Document Type:
Technical Notes
Techniques:
Separation Modes:
Ion-Exchange
Phases: