Documents
Lys C Protease for Improvements in Peptide Mapping Workflows (TN-1269)
Peptide mapping is a ubiquitous method within protein characterization The general workflow includes the isolation of a protein, followed by in solution digest using a serine protease to yield peptides, which are subsequently separated by LC and analyzed by UV and/or MS techniques Because of its specificity and the general size of peptides generated, trypsin is most commonly used However, trypsin can have missed cleavages, particularly with lysine Therefore, a common approach is to supplement the digestion with Lys C, another serine protease In this application note, we demonstrate the sequence coverage results for NIST mAb digestion between standard in solution trypsin when compared to a trypsin/Lys C digest.
Lys C Protease for Improvements in Peptide Mapping Workflows (TN-1269)
Peptide mapping is a ubiquitous method within protein characterization The general workflow includes the isolation of a protein, followed by in solution digest using a serine protease to yield peptides, which are subsequently separated by LC and analyzed by UV and/or MS techniques Because of its specificity and the general size of peptides generated, trypsin is most commonly used However, trypsin can have missed cleavages, particularly with lysine Therefore, a common approach is to supplement the digestion with Lys C, another serine protease In this application note, we demonstrate the sequence coverage results for NIST mAb digestion between standard in solution trypsin when compared to a trypsin/Lys C digest.
Document Type:
Technical Notes
Separation Modes:
Reversed Phase