Selectivity Differences in Size-Exclusion Chromatography for Monoclonal Antibodies; How Pore Size Effects Retention (TN-1251)
The scope of this study is to demonstrate the selectivity differences that can be observed when working with column packing media of different pore sizes. The bioZen SEC-2 media has a 150Å pore and is typically recommended for the separation of smaller mAbs and antibody fragments. The bioZen SEC-3 contains a 300Å pore size that is better for separation of mAb aggregates from monomer. This technote will present the results from this study using two well-characterized mAbs, rituximab and trastuzumab.