Documents
Native MS for Aggregate and Charge Variant Analysis for Biotherapeutics
Size exclusion chromatography (SEC) and ion exchange chromatography (IEX) are classic techniques employed in the characterization of biotherapeutics under non-denaturing conditions. Standard mobile-phase conditions for these techniques are high in salt concentration, rendering them incompatible with mass spectrometry (MS) detection. Native MS represents a cutting-edge approach to employ MS-compatible mobile phases for non-denaturing SEC and IEX analysis. Learn about aggregate and charge-variant analysis using native-mode MS, including understanding the fundamentals in SEC and IEX for biotherapeutic characterization, the effect of mobile-phase conditions and chromatographic parameters in aggregate analysis by native SEC, and determination of acidic and basic variants of monoclonal antibodies using native weak cation exchange chromatography (WCX).
Native MS for Aggregate and Charge Variant Analysis for Biotherapeutics
Size exclusion chromatography (SEC) and ion exchange chromatography (IEX) are classic techniques employed in the characterization of biotherapeutics under non-denaturing conditions. Standard mobile-phase conditions for these techniques are high in salt concentration, rendering them incompatible with mass spectrometry (MS) detection. Native MS represents a cutting-edge approach to employ MS-compatible mobile phases for non-denaturing SEC and IEX analysis. Learn about aggregate and charge-variant analysis using native-mode MS, including understanding the fundamentals in SEC and IEX for biotherapeutic characterization, the effect of mobile-phase conditions and chromatographic parameters in aggregate analysis by native SEC, and determination of acidic and basic variants of monoclonal antibodies using native weak cation exchange chromatography (WCX).
Document Type:
Technical Notes
Techniques:
Brands:
Separation Modes:
Size Exclusion